44 



The Philippine Journal of Science 



1917 



1, EXAMINATION OF THE SERUM OF EXPERIMENTAL CHOLERA CAR- 

 RIERS FOR PRESENCE OF IMMUNE BODIES (AGGLUTININS) 



Guinea pigs and rabbits were infected by intra vesicular injec- 

 tion in the usual way. (3) The guinea pigs were bled from the 

 carotid artery ; the rabbits, from the ear vein. The same strain 

 of cholera vibrio that was used to infect the animals was also 

 employed in performing the agglutination test. The results of 

 these tests are tabulated as follows : 



Table I. — Showing the presence of immune bodies in the serum of experi- 

 mental cholera carriers {guinea pigs). Agglutination test. 



[++, strong agglutination; +. weak agglutination; + tr., trace of agglutination; — , no 



agglutination.] 



CARRIER GUINEA PIGS. 



Guinea 

 pig. 



Days after inoculation. 



Dilutions of serum. 



1/10 



1/20 



1/40 



1/80 



l/i60 



1/320 



1 



2 



3 



4 



6 



6 



7 



8 



9 



10-... 

 11.— 

 12-... 



7 ...... 



+ + 

 + + 

 + + 

 + + 

 + + 

 + + 

 + + 



+ + 

 + + 



+ + 

 + + 



+ + 

 + + 

 + + 



+ + 



+ + 

 + + 



+ + 

 + + 



+ + 

 + + 



+ 



+ 

 + + 

 + + 

 + + 

 + + 

 + ■ 

 + + 

 + + 

 + + 

 + + 



+ 



+ 

 + 

 + 

 + 

 + 

 + 

 + 



+ tr. 





7 



7 



8 



10 .... 



10 - . 



11 .... . . 



11 



11 - . 



11 - . 



16 



16 





NORMAL GUINEA PIGS. 



1 





+ + 

 + + 

 + + 

 + + 

 + + 

 + i- 



+ tr. 

 + tr. 

 + tr. 

 + tr. 

 + tr. 

 + tr. 



— 



- 



— 



— 



2. 





3 





4 





5 





6 .- 









It is evident from Tables I and II that both guinea pigs and 

 rabbits, the latter to a higher degree than the former, show the 

 presence of immune bodies in the blood as a result of intrave- 

 sicular inoculation with cholera vibrios. 



