XII, B, 2 



Schobl and Panganiban: Cholera Garners 



45 



Table II. — Showing the presence of immune bodies in the serum of experi- 

 mental cholera carriers {rabbits). Agglutination test. 



[-I--I-, strong aggrlutination : 4-. weak agglutination; 4- tr., trace of agglutination; — , no 



agglutination.] 



CARRIER RABBITS. 



Rabbit. 



Days after ino- 

 culation. 



Dilutions of serum. 



1/10 



1/20 



1/40 



1/80 



1/160 



1/320 



1/640 



1/1280 



1.-. 

 2.... 

 3.... 

 4.... 



5-..- 



{ 



\ 







4- 

 4- 4- 



4- 

 -i- 4- 



4- 

 4- 4- 



4- 

 4- i- 



4- 

 4- 4- 

 4- 4- 

 4- 4- 

 4- 4- 

 4- 4- 

 -1- 4- 

 4- 4 



4- 



4- 4- 

 ,4- 4- 



r + 



+ + 

 4- 4- 

 4- + 

 4- 4- 

 4- + 

 4- 4- 

 4- 4- 



4- 4- -1- + 4- 4- + 4- -f 

 11+ 1 1 1 1 1 

 + + H- + 4 + + -1- H- 



+ + 4- + + + -1- + + 

 III 1 1 1 1 1 

 4- + + + + H- + + f 



+ + + + ++ + 



III 1 1 + 1 1 + 1 



+ + + + + + + 



+ + + + 



III++ +1 III Ml 

 H- + + + 



M 1 + 1 M + 1 1 1 1 1 1 1 11 





5 . 







6 







7 







10 — 







6 



12 



17. 



22 



28 



38 



49 



57 



The following experiment is included merely for the sake of 

 completeness : 



One rabbit was immunized by intravenous injections of %o> 

 %, %, and 1 slant of heated cholera culture. Twelve days after 

 the last injection a sample of blood was withdrawn from the 

 rabbit's ear vein and about 0.5 cubic centimeter of bile was 

 aspirated from the animal's gall bladder by means of a syringe. 

 Both the blood and the bile, which latter was of normal color and 

 appearance, were subjected to agglutination tests in dilutions of 

 from %o to %2oo- The serum of this rabbit agglutinated cholera 

 vibrios promptly in all dilutions. No agglutination was noticed 

 in the test tubes containing dilutions of the bile. 



One rabbit was immunized by intravenous injections of %o> 

 Vs, 3'2» and 1 slant of heated cholera culture. On the twelfth day 

 after the last injection this animal was infected with %o of a 

 slant of live cholera culture by intravesicular injection. Twenty- 

 four hours after the infection the bile was withdrawn from the 



^ 



