48 



The Philippine Journal of Science 



1917 



III. VACCINE THERAPY OP EXPERIMENTAL CHOLERA CARRIERS 



A series of guinea pigs was infected by intravesicular injec- 

 tion. After the infection some of these animals were vaccinated 

 by subcutaneous injection of heated cholera cultures. From 

 seven to thirteen days after the infection they were killed and 

 examined. The thirteenth day was taken as the upper limit 

 for the reason already given. 



Table IV. — Showing the duration of the state of cholera carriers in treated 



and in untreated animals. 



[+, cholera vibrios found ; — , cholera vibrios not found ; vn, very numerous , more than 200 

 colonies ; n, numerous, about 200 colonies ; f, fevy, about 12 colonies ; vf , very few, less than 

 6 colonies.] 



Gui- 

 nea 

 pig- 



Treatment. 



Mode of 

 immuniza- 

 tion. 



li 



j^ 

 '■A 



7 



3 



7 



9 



9 



10 



11 



11 



12 



4^ 

 00 

 CS 



o.S 



r 



Q 

 6 



2 

 1 

 6 

 3 



4 

 4 

 4 



Direct plates. 



Peptone cultures. 



Days 

 after 

 infec- 

 tion. 



Dose of vac- 

 cine in 

 slants. 



_3J 



n 



r 

 3 C 



Q 



s 

 1 



S 



a 



u 



CO 



s a) 

 O 





 Q 



1 



1— 1 



s 

 o 



1 



2 

 3 



4 

 5 

 6 

 7 

 8 

 9 



1 



1 

 6 

 1 

 1 

 4 

 4 

 4 

 1 



1/10 



Subcuta- 

 neous, 

 do 



vn 



vn 

 vn 



f 



n 

 vf 



n 



f 

 vn 



n 



vn 

 vf 

 n 



vn 



f 



vn 

 vn 



n 



vf 

 vf 



t 

 f 



-f 



+ 

 + 

 + 

 + 

 +- 

 -1- 



. + 



+ 

 + 

 + 



+ 



+ 



+ 



+ 

 + 

 + 

 + 

 + 

 + 



+ 

 + 



H- 



1/10 



1/10 - - 



..__do 



1/10, 1/5, l/2_ 

 1/10, 1/5, l/2_ 

 1/10,1/5 



....do 



..-.do 



.__.do 



1/10,1/5 



___.do 



1/10,1/5 



.___do 



1/10,1/5 



.._.do 



UNTREATED ANIMALS. 



n 

 vn 



n 



vn 

 vn 



vf 

 n 



f 



f 



vf 



n 



n 



+ 

 + 

 H- 

 -I- 



4 

 + 

 + 



+ I + 



+ — 



These tables show the results of experiments concerning the 

 influence of immunization upon the state of experimental cholera 

 carriers. Unless we interpret as an effect of immunization the 

 one negative animal (guinea pig 13, Table III) and the apparent 

 decrease of cholera vibrios in the intestinal canal, as indicated 

 by absence of cholera colonies on direct plates, we fail to see 

 any effect of immunization upon the cholera carrier state in 

 experimental animals. 



