THE INFLUENCE OF BILE UPON THE DISTRIBUTION OF 

 CHOLERA VIBRIOS IN THE DIGESTIVE SYSTEM OF 

 y"' EXPERIMENTAL CHOLERA CARRIERS^ 



By Otto Schobl 

 {From the Biological Laboratory, Bureau of Science, Manila) 



The distribution of cholera vibrios in the alimentary canal of 

 experimental cholera carriers has been studied in the early 

 experiments.- It was found that the excretion of cholera vibrios 

 in the faeces of successfully infected animals is irregular and 

 not as frequent as one would expect. Out of 26 samples of 

 faeces taken from 26 guinea pigs, only 2 were found to contain 

 cholera vibrios at the time of examination. In another experi- 

 ment a systematic examination of faeces for the presence of 

 cholera vibrios was made in 18 guinea pigs. Out of 71 examina- 

 tions of faeces taken from these animals at various times, only 

 14 tests were positive. 



During the studies on the influence of bile upon the state of 

 cholera carriers in experimental animals,^ it was discovered ac- 

 cidentally that animals fed on bile excreted cholera vibrios in 

 their faeces more frequently than normal cholera carriers. This 

 finding was considered important enough to be further studied. 



A series of guinea pigs was infected by intravesicular injec- 

 tion. Some of the animals were fed on bile ; the remainder were 

 kept as normal controls. They were killed and examined for 

 the presence of cholera vibrios at intervals. Cultures were made 

 from the gall bladder, duodenum, ileum, caecum, and faeces. Both 

 direct Dieudonne's plate and peptone cultures were planted.* 



' Received for publication October 5, 1916. 



'Journ. Inf. Dis. (1916), 18, 307-314; (1916), 19, 145-152. 



'This Journal, Sec. B (1916), 11, 157. 



* For details of technic, see Joum. Inf. Dis. (1916), 18, 307-314. 



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