xn.B,B Schobl: Survey of Certain Chemicals 217 



immediately visible, and prolapsing into the laparotomy wound, 

 it closed the opening, thus preventing exposure of the other 

 organs. Therefore the injection could be made outside the peri- 

 toneal cavity. Only fractions of a cubic centimeter were injected 

 into the gall bladder. The inoculation finished, the puncture in 

 the gall bladder was closed with a ligature, the ends of the wound 

 were lifted up, and the gall bladder assumed its normal position. 

 Threads thoroughly soaked with tincture of iodine were used for 

 suturing the abdominal wound. 



Examination. — To ascertain the effect of the drug, the animals 

 were killed and examined within several hours after the last ad- 

 ministration of the drug. The shaved skin over the chest and ab- 

 domen was wetted with 2 per cent lysol solution. The abdomen 

 and thorax were opened separately. The gall bladder, the proxi- 

 mal part of the intestine (in the reports, duodenum), the distal 

 part of the small intestine (in the reports, the ileum) , and the 

 caecum were removed from the abdomen — first the gall bladder, 

 then the duodenum, ileum, caecum, in order, a separate set of 

 sterilized instruments being used for each organ. The gall blad- 

 der was taken out in tbto, including a portion of the bile duct. 

 The contents of the gall bladder were emptied into peptone water, 

 and the gall bladder, together with the bile duct, was placed in a 

 culture tube. The contents of the proximal part of the small gut 

 were planted, and the intestine, cut into small pieces, was added 

 to the same peptone tube. At least one half of the distal part 

 of the small intestine was planted in the same way as the 

 duodenum. Five large loopfuls of the contents of the caecum 

 were inoculated into peptone water. One loopful of the contents 

 of the various organs mentioned above was plated directly on 

 Dieudonne agar plates. After six hours of incubation second 

 peptone cultures were planted from the first peptone tubes. At 

 the end of twenty-four hours Dieudonne plates were made from 

 the first and the second peptone cultures. 



It was the purpose of these experiments to test as many 

 chemicals as possible in the hope that some of them would show 

 an indication of curative eft'ect. For this reason comparatively 

 few animals were treated with each drug, the intention being to 

 extend the experiments later on, using those chemicals which 

 showed promising results in the present investigations. 



As to the dosage of the drugs small doses were given at the 



start. The quantity of the drug was gradually increased until 



toxic symptoms — even death — occurred as a consequence of the 



drug feeding. It was intended to saturate the body organism of 



■ the animals with the particular drug. In order to see the imme- 



