218 ^he Philippine Journal of Science 1917 



diate effect of the drug upon the cholera vibrios, particularly 

 in the intestine, the animals were killed within a few hours 

 after the last feeding instead of awaiting the time when a com- 

 plete elimination of the chemical from the animal body could 

 be reasonably expected. 



The objection to such a procedure, namely, the possibility of 

 transferring into the culture medium an amount of the drug 

 sufficient to inhibit the growth of cholera vibrios in the cultures, 

 was kept in mind, but it soon became evident that this factor 

 hardly warranted consideration. Numerous experiments with 

 various chemicals were made in this way without any apparent 

 curative effect of the drugs being noticeable. Furthermore the 

 present investigation was intended for orientation only, and those 

 drugs which showed any effect at all were to be further studied. 



The results of our experiments are summarized in Tables I 

 to VIII. From ten to twelve animals were inoculated and treated 

 at the same time, one of the carriers, at least, being kept as 

 a control. The administration of the drugs took place either by 

 intramuscular injection or by mouth. For the latter way of 

 drug application an inert oil was found technically more con- 

 venient than water. The animals under treatment were killed 

 not later than the tenth day, provided they survived the treat- 

 ment that long. It has been found in our previous experiments 

 that a certain percentage of experimental cholera carriers be- 

 come spontaneously negative after the thirteenth day. There- 

 fore the duration of treatment had to be limited to ten days. 



For the sake of convenience we have arranged the chemicals 

 and drugs that were used in the present experiments into seven 

 groups. 



The first group contains simple organic compounds : Benzene, 

 toluene, and xylene. 



The second group contains halogen derivatives: Chloroform, 

 bromoform, and chloral. 



The third group represents phenols and related compounds: 

 Salicylates, benzoic acid, resorcin, brenzkatechin, guaiacol, gallic 

 acid, pyrogallol, carvacrol, thymol, creosote, xylenol, and alpha- 

 and beta-naphthol. 



The fourth group contains miscellaneous organic compounds, 

 the majority of which are official in the pharmacopoeia : Urotro- 

 pin, 01. caryophylli, 01. cinamoni, 01. copaibse, camphor, eucalyp- 

 tol, anethol, phenetol, terpineol, and turpentine. 



Two alkaloids, namely, quinine and emetine, were tried also 

 because of their usefulness in chemotherapy. (The fifth group.) 



Certain representatives of the officinal compounds of metals 



