XII, B, 6 Boynton: Diseases in Cattle 285 



MICROSCOPIC PICTURE OP THE BLOOD (PLATES II AND III) 



As was stated, the blood was very anaemic, which made it 

 difficult to obtain good, even smear preparations. 



In stained preparations the red cells were lacking in haemo- 

 globin and had a great tendency to become crenate, irrespective 

 of the precautions taken to prevent this. As a rule, the bodies 

 were situated on the margins of the corpuscles. In some in- 

 stances they protruded over or at least caused the corpuscle to 

 bulge from the margin. Where two bodies were present in 

 one corpuscle, frequently one was situated on the periphery, 

 while the other would be nearer the center, in the center, or 

 beyond the center; in some instances they would be on the 

 periphery of the corpuscle, one on either side. This indicates 

 that frequently after division one of the newly formed bodies 

 remains at or near its original location, while the other migrates 

 to the other side of the corpuscle. In other cases they divide, 

 and both remain at the periphery on the same side of the 

 corpuscle. 



Frequently the newly formed bodies after division are of equal 

 size ; however, the reverse is common in which one may be much 

 larger than the other. A few instances were noted where divi- 

 sion was not complete, one being as much as three times the 

 size of the other, the smaller giving the appearance of a bud 

 protruding from the larger, similar to the condition noticed in 

 yeast cells undergoing multiplication. 



As a rule, one or two bodies were found in an infected cell, but 

 four or even five in a cell were not of rare occurrence. 



With Giemsa's stain the bodies become a purplish red, staining 

 very brilliantly, and are easy to distinguish. They vary in size 

 from 0.5 micron to 1.5 microns. 



They stained uniformly and appeared to be composed of a mass 

 of chromatin with no cellular substance. A slight halo is pre- 

 sent around a majority of the bodies, which may be due to 

 the lack of haemoglobin in their immediate vicinity, but this 

 halo does not have the appearance of a lighter staining body 

 substance. 



With Wright's stain the bodies appear smaller than with 

 Giemsa's method. It may be possible that with Wright's method 

 all the chromatin is not stained; however, a body structure 

 could not be distinguished. 



Morphologically they are spherical or have a slightly uneven 

 border, thus making any definite shape impossible, but there 

 is a general tendency toward being spherical. Undoubtedly 



