The Philippine Journal of Science 



1916 



Table II'. — The influence of formalin on the Tschemoguhow modification of 



the Wassermann reaction. 



No. of 

 serum. 



Date 

 secured. 



Por- 

 tion. 



Date 

 heated. 



Date 



tested. 



Se- 

 rum. 



Tube- 



Result. 



1 



2 



3 



4 



5 





 

 

 

 

 

 

 

 

 

 

 



1' 



+ 

 + 



+ 

 + 



+ 

 + 

 + 

 + 

 + 

 + 

 + 



2' 



+ 

 + 

 + 

 + 

 + 

 + 

 + 

 -t- 

 -1- 

 -t- 

 -1- 

 + 



3' 



tr 



+ 

 + 

 + 

 + 

 + 

 + 

 + 

 -1- 

 + 

 + 

 + 



4' 





 

 tr 

 

 

 

 

 tr 

 

 

 

 



5' 





 

 

 

 

 

 

 

 

 

 

 



4401 

 4403 

 4404 

 4405 



1915. 

 Oct. 29 



-..do .... 



...do.... 



...do .... 



f A 



B 



I B 



1b 



f A 



1b 



B 



1915. 



1915. 

 Oct. 30 

 Nov. 7 



...do .... 

 Oct. 30 

 Nov. 7 



...do.... 

 Oct. 30 

 Nov. 7 



...do.... 

 Oct. 30 

 Nov. 7 



...do .... 



cc. 



0.1 

 0.1 



0.1 

 0.1 



0.1 

 0.1 



0.1 

 0.1 



+ 

 + 



T 







+ 

 + 

 + 

 + 



+ 

 + 







+ 

 + 

 

 + 

 + 

 



+ 

 -1- 

 + 

 + 

 + 







± 

 + 

 



+ 







+ 

 ± 







± 

 + 





 

 tr 

 

 

 

 

 

 

 

 

 



Moderately positive. 

 Weakly positive. 

 Negative. 

 Strongly positive. 

 Weakly positive. 

 Negative. 

 Strongly positive. 

 Weakly positive. 



Do. 

 Moderately positive. 

 Weakly positive. 

 Negative. 





Nov. 7 





Nov. 7 





Nov. 7 





Nov. 7 



Tables II and IP show the results obtained by testing for- 

 malinized serum by the Wassermann method and by the Tschern- 

 ogubow modification. Some of these sera gave strongly positive 

 results before formalin had been added. With the unheated 

 sera weakly positive results were obtained a week after the 

 formalin had been added. After having been heated to 55° C. 

 for thirty minutes, all but one serum gave negative results. 

 Unheated, these sera were but slightly anticomplementary a 

 week after having been secured ; less so for the Tschernogubow 

 modification than for the Wassermann method. All sera were 

 bacteriologically sterile. 



TEST 3 



The influence of glycerin on the Wassermann reaction. — Speci- 

 mens 4318, 4319, 4320, 4321, 4322, 4326, 4328, 4335, 4336, and 

 4338 were secured on September 29, 1915. On September 30, 

 1915, the sera were drawn ofi: the clots. Each serum was 

 divided into two portions, A and B. Portion A was tested by 

 the Wassermann method on September 30, 1915, without having 

 been mixed with glycerin. Portion B was mixed with an equal 

 volume of sterilized, neutral glycerin, was kept at room tem- 

 perature in a cork-stoppered test tube, and was tested at inter- 

 vals of a week or more. Each serum was examined for 

 bacterial contamination. 



