8 



The Philippine Journal of Science 



1916 



Table III. — The influence of glycerin on the Wassermann 

 reaction — Continued. 



No. of 

 serum. 



Date 

 secured. 



Por- 

 tion. 



Date 

 heated. 



Date 

 tested. 



Quan- 

 tity 

 of se- 

 rum. 



Am- 

 bo- 

 cep- 

 tor. 



Tube— 



Result. 



1 



2 



3 



1' 



2' 



3' 





1915. 





1915. 



1915. 



cc. 



Unit. 





















A 



Sept. 30 



Sept. 30 



0.1 



1.25 



+ 



-1- 







-1- 



+ 



± 



Moderately pos- 

 itive. 



4336 



-__do.... 



B 



Oct. 7 



Oct. 7 



0.1 



1.25 



+ 



± 







-1- 



-f 



tr 



Do. 



B 



Oct. 14 



Oct. 14 



0.1 



1.25 



tr 











-T 



tr 







Strongly positive. 







B 



Oct. 24 



Oct. 24 



0.1 



1.25 



tr 











+ 



tr 







Do. 







B 





...do.— 



0.1 



1.25 



























Anticomple m e n - 

 tary. 







A 



Sept. 30 



Sept. 30 



0.1 



1.25 



+ 



± 







-1- 



+ 



+ 



Strongly positive. 







B 



Oct. 7 



Oct. 7 



0.1 



1.25 



+ 











+ 



+ 



tr 



Do. 



4338 



...do .... 



B 



Oct. 14 



Oct. 14 



0.1 



1.25 



tr 











-1- 



± 







Do. 







B 



Oct. 24 



Oct. 24 



0.1 



1.25 















+ 



tr 







Do. 







B 





...do .... 



0.1 



1.25 



























Anticompl e m e n- 

 tary. 







Table III shows the results obtained in testing glycerinated 

 sera 4318, 4319, 4320, 4321, 4322, 4326, 4328, 4335, 4336, and 

 4338 by the Wassermann method. Glycerin did not noticeably 

 influence this method. The results obtained by this test seven 

 days, fourteen days, and twenty-four days after the sera had 

 been mixed with glycerin were practically identical with the 

 results obtained before the sera had been mixed with glycerin. 

 It seems that sera that gave a positive result while fresh became 

 more strongly positive with age. Negatives remained negative. 

 Unheated, all sera were strongly anticomplementary on the 

 twenty-fourth day after they had been mixed with glycerin; 

 heating the sera to 55° C. for thirty minutes did not entirely 

 destroy the anticomplementary property. All sera remained 

 free from bacterial growth. 



TEST 4 



The influence of glycerin on the Wassermann reaction. — On 

 October 13, 1915, specimens 4360, 4361, 4364, 4365, 4366, 4369, 

 4370, 4371, 4372, and 4374 were secured. The sera were drawn 

 off the clots on October 14, 1915. Each serum was divided into 

 two portions, A and B. Portion A, unglycerinated, was tested 

 by the Wassermann method on October 14, 1915. Portion B 

 was mixed with an equal volume of sterilized, neutral glycerin, 

 was kept at room temperature in a cork-stoppered test tube, and 

 was tested by the Wassermann method at intervals of about a 

 week. A bacteriologic examination was made of each serum. 



