10 



The Philippine Journal of Science 



1916 



Table IV. — The influence of glycerin on the Wassermann 

 reaction — Continued. 



No. of 

 serum. 



Date 

 secured. 



Por- 

 tion. 



Date 

 heated. 



Date 



tested. 



Se- 

 rum. 



Am- 

 bo- 

 cep- 

 tor. 



Tube— 



Result. 



1 



2 



3 



1' 



2- 



3' 





1915. 





19J5. 



1915. 



ce. 



Unit. 





















A 



Oct. 14 



Oct. 14 



0.1 



1.0 



+ 



+ 







+ 



+ 



± 



Moderately p o s - 

 itive. 







B 



Oct. 24 



Oct. 24 



0.1 



1.0 



± 











+ 



tr 







Do. 



4372 



Oct. 13 



B 





...do .... 



0.1 



1.0 



























Anticomple m e n - 

 tary. 







B 



Oct. 31 



Oct. 31 



0.1 



1.0 



4: 











+ 



tr 







Moderately p o s - 

 itive. 







B 



Nov. 7 



Nov. 7 



0.1 



1.0 



± 











+ 



tr 







Do. 







A 



Oct. 14 



Oct. 14 



0.1 



1.0 



+ 



4; 







-f 



+ 



tr 



Do. 







B 



Oct. 24 



Oct. 24 



0.1 



1.0 



± 











+ 



tr 







Do. 



4374 



...do .... 



B 





...do.... 



0.1 



1.0 



























Anticomple m e n - 

 tary. 







B 



Oct. 31 



Oct. 31 



0.1 



1.0 



± 











+ 



tr 







Moderately p o s - 

 itive. 







B 



Nov. 7 



Nov. 7 



0.1 



1.0 



± 











+ 



tr 







Do. 



4370 



...do .... 



B 



...do.... 



...do.... 



0.1 



1.5 



± 











+ 



+ 







Strongly positive. 



4372 



...do .... 



B 



...do.-.. 



...do.... 



0.1 



1.5 



+ 



tr 







+ 



+ 







Moderately pos- 

 itive. 



4374 



...do.... 



B 



...do.... 



...do...- 



0.1 



1.5 



+ 











+ 



+ 







Strongly positive. 



The result obtained with sera 4360, 4361, 4364, 4365, 

 4366, 4369, 4370, 4371, 4372, and 4374 by the Wassermann 

 reaction are shown in Table IV. The glycerinated portion of 

 each of these sera gave results practically identical with the 

 result obtained with the nonglycerinated portion. Unheated, 

 nine of these ten sera were strongly anticomplementary on the 

 tenth day, after they had been mixed with glycerin. One serum, 

 No. 4364, had not become anticomplementary. The anticom- 

 plementary property was not entirely destroyed by heating the 

 sera to 55° C. for thirty minutes, and it did not alter the result 

 of the test. Such anticomplementary property was overcome 

 by increasing the quantity of amboceptor. All sera were sterile. 



TEST 5 



The influence of glycerin on the Tschernogubow modification 

 of the Wassermann reaction. — Specimens 4361, 4365, 4366, and 

 4369 were secured on October 13, 1915. The sera were drawn 

 off the clots on October 14, 1915. Each serum was divided into 

 two portions, A and B. Unheated and unglycerinated, portion 

 A was tested on October 14, 1915. Portion B was mixed with 

 an equal volume of sterilized, neutral glycerin, was kept at 

 room temperature in a cork-stoppered test tube, and was tested 

 two weeks later. 



