XI, B, 1 Ruediger: Preservation of Human Serum H 



Table V. — The influence of glycerin on the Tschemogubow modification. 



No. of 

 serum. 



Date 



secured. 



Por- 

 tion. 



Date 

 heated. 



Date 

 tested. 



Se- 

 rum. 



Tube— 



Result. 



1 



2 



3 



4 



6 



r 



2' 



3' 



4' 



5' 



4361 

 4365 

 4366 

 4369 



1915. 

 Oct. 13 



...do.... 



...do.... 



...do.... 



J •*■ 

 1 B 



A 



B 



A 



B 



A 



B 



1915. 



1915. 

 Oct. 14 

 Oct. 31 

 Oct. 14 

 Oct. 31 

 Oct. 14 

 Oct. 31 

 Oct. 14 

 Oct. 31 



cc. 

 0.1 

 0.1 

 0.1 

 0.1 











+ 

 + 

 + 

 + 

 



+ 





 



+ 

 + 

 + 

 + 

 

 





 



± 

 + 

 + 

 + 

 

 





 

 

 

 

 

 

 





 

 

 

 

 

 

 



+ 

 + 

 + 

 + 

 + 

 + 

 + 

 + 



+ 

 + 



+ 



+ 

 + 

 + 



+ 

 tr 



+ 

 + 

 + 

 + 

 + 





 

 

 

 

 

 ± 

 





 

 

 



« 









 



Strongly positive. 



Do. 

 Negative. 



Do. 



Do. 



Do. 

 Strongly positive. 



Do. 



Oct. 31 



Oct. 31 



Oct. 31 



Oct. 31 



Table V shows that with these four sera glycerin did not in- 

 fluence the Tschemogubow modification of the Wassermann 

 reaction. 



TEST 6 



The influence of glycerin on the Wassermann reaction. — Spec- 

 imens 4394, 4395, 4396, 4897, 4398, and 4399 were secured on 

 October 27, 1915, and the sera were drawn off the clots the next 

 day. Each serum was divided into four portions — A, B, C, 

 and D. Unglycerinated, portion A was heated and tested by 

 the Wassermann method on October 28, 1915. Portion B was 

 mixed with an equal volume of sterilized, neutral glycerin and 

 was heated to 55° C. for thirty minutes on October 28, 1915; 

 was tested by the Wassermann method on November 7, 1915. 

 Portion C was mixed with an equal volume of sterilized, neutral 

 glycerin on October 28, 1915, and was heated and tested by the 

 Wassermann method on November 7, 1915. D was mixed with 

 an equal volume of sterilized, neutral glycerin on October 28, 

 1915, and without having been heated, was tested by the Was- 

 sermann method on November 7, 1915. Each serum was exam- 

 ined bacteriologically. 



Table VI shows the results obtained with glycerinated sera 

 4394, 4395, 4396, 4397, and 4398, tested by the Wassermann 

 method. The glycerin did not noticeably influence the Wasser- 

 mann reaction. Glycerinated sera that were heated on October 

 28, 1915, and were tested on November 7, 1915, were not more 

 anticomplementary than were sera that were heated and tested 

 on November 7, 1915. Unheated, five of the six sera were 

 strongly anticomplementary on November 7, 1915. Serum 4399 

 was no more anticomplementary before having been heated than 

 it was after it had been heated on November 7, 1915. 



