12 The Philippine Journal of Science 1916 



Table VI. — The influence of glycerin on the Wassermann reaction. 



No. of 

 serum. 



Date 

 secured. 



Por- 

 tion. 



Date 

 heated. 



Date 

 tested. 



Se- 

 rum. 



Am- 

 bo- 

 cep- 

 tor. 



Tube- 



Result. 



1 



2 



3 



1' 



2' 



3' 





1915. 





1915. 



1915. 



cc. 



Unit. 





















r ^ 



Oct. 28 



Oct. 28 



0.1 



1.0 



+ 











+ 



+ 







Moderately posi- 

 tive. 



4394 



Oct. 27 



B 

 C 



...do.-.. 



Nov. 7 



Nov. 7 

 ...do .... 



0.1 

 0.1 



1.0 

 1.0 



± 

 tr 





 





 





tr 

 tr 





 



Do. 

 Do. 







D 





...do .... 



0.1 



1.0 



























Anticomplemen- 





























tary. 







A 



Oct. 28 



Oct. 28 



0.1 



1.0 



+ 



± 







+ 



± 







Negative. 







B 



—do .... 



Nov. 7 



0.1 



1.0 



+ 



tr 







+ 



tr 







Do. 



4395 



...do..- 



C 



Nov. 7 



...do .— 



0.1 



1.0 



+ 



tr 







+ 



tr 







Do. 







D 





...do—. 



0.1 



1.0 



























Anticomplemen- 





























tary. 







A 



Oct. 28 



Oct. 28 



0.1 



1.0 



+ 











+ 



H- 







Strongly positive. 







B 



...do .... 



Nov. 7 



0.1 



1.0 



tr 











+ 



tr 







Do. 



4396 



...do.... 



C 



Nov. 7 



... dc... 



0.1 



1.0 



tr 











+ 



tr 







Do. 







D 





...do.... 



0.1 



1.0 



























Anticompl e m e n- 

 tary. 







A 



Oct. 28 



Oct. 28 



0.1 



1.0 



tr 











+ 



± 







Strongly positive. 



4397 



...do .... 



B 

 C 



...do.— 

 Nov. 7 



Nov. 7 

 ...do.... 



0.1 

 0.1 



1.0 

 1.0 





 





 





 



+ 



tr 

 





 



Do. 

 Do. 







D 





...do.... 



0.1 



1.0 



























Anticomplemen- 

 tary. 







A 



Oct. 28 



Oct. 28 



0.1 



1.0 



± 











+ 



± 







Strongly positive. 



4398 



...do.... 



B 

 C 



...do.... 

 Nov. 7 



Nov. 7 

 —do.... 



0.1 

 0.1 



1.0 

 1.0 



tr 

 tr 





 





 



+ 



tr 

 tr 





 



Do. 

 Do. 







D 





_..do.-.. 



0.1 



1.0 



























Anticomplem e n - 





























tary. 







A 



Oct. 28 



Oct. 28 



0.1 



1.0 



+ 



± 







+ 



+ 







Negative. 



4399 



...do...- 



B 

 C 



...do .... 

 Nov. 7 



Nov. 7 

 —do.-.. 



0.1 

 0.1 



1.0 



1.0 



+ 

 + 



tr 

 tr 





 



+ 



tr 

 tr 





 



Do. 

 Do. 







D 





...do.... 



0.1 



1.0 



+ 



tr 







+ 



tr 







Do. 



TEST 7 



The influence of glycerin on the Tschernogubow modification 

 of the Wassermann reaction. — Specimens 4394, 4395, and 4396 

 were secured on October 27, 1915. The sera were drawn off 

 the clots on October 28, 1915. Each serum was divided into 

 three portions — A, B, and C. Portion A, unglycerinated and 

 unheated, was tested by the Tschernogubow modification on 

 October 28, 1915. Portion B was heated to 55° C. for thirty 

 minutes and was then mixed with an equal volume of sterilized, 

 neutral glycerin. On November 7, 1915, portion B was sub- 

 divided into portions B and B'. Portion B was tested without 

 further heating, and portion B' was tested after it had been 

 reheated. Unheated, portion C was mixed with an equal volume 

 of sterilized, neutral glycerin on October 28, 1915. On Novem- 



