XI, B, 1 



Ruediger: Preservation of Human Serum 



13 



ber 7, 1915, portion C was subdivided into two portions, C and 

 C Portion C was tested without having been heated, and por- 

 tion C was tested after it had been heated to 55° C. for thirty 

 minutes. 



Table VII. — The influence of glycerin on the Tschernogubow modification 



of the Wassermann reaction. 



No. 

 of se- 

 rum. 



Date se- 



Por- 



Date 

 heated. 



Date re- 

 heated. 



Date 



tested. 



Se- 

 rum. 



Tube— 



Result. 



cured. 



tion. 



1 



2 



3 



4 



5 



1' 



2' 



3' 



4' 



5' 





1915. 





1915. 



1915. 



1915. 



cc. 





























A 

 B 







Oct. 28 

 Nov. 7 



0.1 



-r 



+ 





 tr 





 





 





 



-t- 



+ 



+ 

 -t- 



+ 

 tr 





 





 



Moderately positive. 

 Do. 



Oct. 28 





4394 



Oct. 27 



B' 



-.do... 



Nov. 7 



..do — 



0.1 



+ 



tr 















+ 



+ 



± 











Do. 







C 







-.do ... 



0.1 



tr 



















+ 



tr 















Do. 







C 



Nov. 7 





..do... 



0.1 



+ 



tr 















+ 



-t- 



± 











Do. 







A 

 B 







Oct. 28 

 Nov. 7 



""OA 



+ 

 + 



+ 

 + 



+ 



tr 







n 





 



-f- 



+ 



-1- 

 -f- 



-1- 

 tr 





 





 



Negative. 

 Do. 



Oct. 28 





4395 



..do... 



B' 



..do ... 



Nov. 7 



..do ... 



0.1 



+ 



j- 



± 



n 







+ 



+ 



± 











Do. 







C 



C 







. do... 

 ..do... 



0.1 

 0.1 



+ 

 -1- 



tr 



+ 







± 







n 





 



+ 

 + 



tr 



4- 







± 





 





 



Do. 

 Do. 



Nov. 7 









A 

 B 







Oct. 28 

 Nov. 7 



0.1 



+ 

 + 





 













 





 



+ 



+ 



+ 

 + 



+ 



± 



+ 















Strongly positive. 

 Do. 



Oct. 28 





4396 



..do ... 



B' 



..do ...Nov. 7 



-do ... 



0.1 



± 



















+ 



+ 



± 











Do. 







C 





..do... 



0.1 























+ 



+ 















Do. 







C 



Nov. 7 



..do... 



0.1 + 



















+ 



+ 



± 











Do. 



As is shown in Table VII, glycerin did not influence the 

 Tschernogubow modification of the Wassermann reaction. 

 Heating the sera to 55° C. for thirty minutes more than once 

 did not alter the results obtained, and unheated, these sera had 

 become but slightly anticomplementary for the Tschernogubow 

 modification of the Wassermann reaction in eleven days. 



TEST 8 



The influence of glycerin on the Tschernogubow modification 

 of the Wassermann reaction. — Specimens 4397, 4398, and 4399 

 were secured on October 27, 1915. On the next day the 

 sera were drawn off the clots. Each serum was divided into 

 two portions, A and B. Unglycerinated and unheated, portion 

 A was tested by the Tschernogubow modification of the Wasser- 

 mann reaction on October 28, 1915. Portion B was mixed with 

 an equal volume of sterilized, neutral glycerin and was kept 

 in a cork-stoppered test tube at room temperature. On No- 

 vember 7, 1915, portion B was tested by the Tschernogubow 

 modification before and after the serum had been heated to 55° 

 C. for thirty minutes. 



