14 



The Philippine Journal of Science 



1916 



Table VIII. — The influence of glycerin on the Tschemogubow modification of 



the Wassermann reaction. 



No. of 

 serum. 



Date 

 secured. 



Por- 

 tion. 



Date 

 heated. 



Date 



tested. 



Se- 

 rum. 



Tube— 



Result. 



1 



2 



3 



4 



5 



1' 



+ 

 + 

 + 

 + 

 + 

 + 



+ 

 + 



2' 



+ 

 + 

 + 

 + 

 + 

 + 

 ± 

 + 



3' 



-^ 

 



± 



+ 







-1- 



4- 

 ~ 



4' 



+ 

 

 

 



± 





 

 



5' 





 

 





 

 

 

 



4397 

 4398 

 4399 



1915. 

 Oct. 27 



...do .... 



...do .... 



A 



Ib 



A 



1 ^ 

 I B 



A 



B 



I B 



1915. 



1915. 

 Oct. 28 

 Nov. 7 



...do .... 

 Oct. 28 

 Nov. 7 



...do .— 

 Oct. 28 

 Nov. 7 



...do.... 



cc. 



0.1 

 0.1 



0.1 

 0.1 



0.1 

 0.1 





 



+ 







± 



+ 



+ 

 + 

 + 





 

 

 

 

 + 

 + 

 ± 

 + 





 

 

 

 

 tr 

 ± 

 

 ± 





 

 

 

 

 

 

 

 





 

 

 

 

 

 

 

 



Strongly positive. 



Do. 



Do. 



Do. 



Do. 



Do. 

 Negative. 



Do. 



Do. 





Nov. 7 





Nov. 7 





Nov. 7 



Table VIII shows that in test 8 the glycerin did not influence 

 the Tschemogubow modification of the Wassermann reaction. 

 Unheated, these three sera were moderately anticomplementary 

 on November 7, 1915. Heating the sera to 55° C. for thirty 

 minutes destroyed the anticomplementary property. 



TEST 9 



The appearance of anticomplementary properties. — Specimens 

 4557, 4558, 4559, 4560, and 4561 were secured on January 4, 

 1916. On January 5, 1916, the sera were drawn off the clots. 

 Each serum was divided into seven portions — A, B, C, D, E, 

 F, and G. Unglycerinated, portion A was tested by the Wasser- 

 mann method on January 5, 1916. Portion B was heated to 

 55° C. for thirty minutes, was mixed with an equal volume of 

 sterilized, neutral glycerin, and was kept at room temperature 

 in a cork-stoppered test tube. Portions C, D, E, F, and G 

 were mixed with equal volumes of sterilized, neutral glycerin 

 and were kept at room temperature in cork-stoppered test tubes. 

 Beginning with January 5, 1916, one portion of glycerinated 

 serum was heated to 55° C. for thirty minutes on every third 

 day until all the portions had been heated. Portion C was 

 heated on January 5; portion D, on January 8; portion E, on 

 January 11 ; portion F, on January 14 ; and portion G, on January 

 16, 1916. On the last day portions B, C, D, E, F, and G were 

 tested by the Wassermann method and a bacteriologic test was 

 made of each serum. 



