XI. B, 1 Ruediger: Preservation of Human Serum 15 



Table IX. — The appearance of anticomplementary properties. 



No. of 

 serum' 



Date 

 secured. 



Por- 

 tion. 



Date 

 heated. 



Date 

 tested. 



Se- 

 rum. 



Am- 

 bo- 

 cep- 

 tor. 



Tube— 



Result. 



1 



2 



3 



1' 



2' 



3' 





1 

 1916. 



1916. 



1916. 



cc. 



Unit. 





















A 



Jan. 5 



Jan. 5 



0.1 



1.0 



-1- 



+ 



tr 



-1- 



4- 



tr 



Negative. 







B 



...do .... 



Jan. 16 



0.1 



1.0 



+ 



+ 







-1- 



4- 







Do. 







C 



...do.... 



...do.... 



0.1 



1.0 



























Anticomple m e n - 



4657 



Jan. 4 



D 



Jan. 8 



...do .... 



0.1 



1.0 



-1- 



tr 







4 



tr 







tary. 

 Negative. 







E 



Jan. 11 



...do..-. 



0.1 



1.0 



+ 



tr 







+ 



tr 







Do. 







F 



Jan. 14 



...do .... 



0.1 



1.0 



4- 



± 







+ 



± 







Do. 







G 



Jan. 16 



...do.... 



0.1 



1.0 



+ 



+ 







+ 



+ 







Do. 





; 



A 



Jan. 5 



Jan. 5 



0.1 



1.0 



+ 



-t- 



tr 



+ 



4- 



tr 



Do. 





i 



B 



...do Jan. 16 



0.1 



1.0 



+ 



+ 







+ 



4- 







Do. 





i 



C 



...do |...do 



0.1 



1.0 



± 











± 











Do. 



46S8 



...do.... 



D 



Jan. 8 !...do 



0.1 



1.0 



+ 











4- 











Do. 







E 



Jan. 11 ...do 



0.1 



LO 



■+ 



tr 







4- 



tr 







Do. 





1 



F 



Jan. 14 



...do .... 



0.1 



1.0 



+ 



4: 







4- 



± 







Do. 





j 



G 



Jan. 16 



...do.... 



0.1 



1.0 



■+ 



+ 







4- 



4- 







Do. 







A 



Jan. 5 1 Jan. 5 



0.1 



1.0 



+ 



-1- 



tr 



4- 



4- 



tr 



Do. 







B 



...do ....' Jan. 16 



0.1 



1.0 



+ 



-r 







-1- 



4- 







Do. 





j 



C 



...do do .... 



0.1 



1.0 



tr 











tr 











Do. 



4559 



...do .... 



D 



Jan. 8 ...do 



0.1 



1.0 



-1- 











4- 











Do. 







E 



Jan. 11 ...do 



0.1 



1.0 



+ 



tr 







4- 



tr 







Do. 







F 



Jan. 14 ...do 



0.1 



1.0 



+ 



± 







4- 



± 







Do. 







G 



Jan. 16 ...do 



0.1 



1.0 



+ 











4- 











Do. 







A 



Jan. 5 



Jan. 5 



0.1 



1.0 



+ 



+ 







4- 



4- 



tr 



Moderately posi- 

 tive. 







B 



...do..- 



Jan. 16 



0.1 



1.0 



-r 











4- 



4- 







Strongly positive. 



4660 



...do .... 



C 

 D 



...do — . 

 Jan. 8 



...do .... 

 ...do .... 



0.1 

 0.1 



1.0 

 LO 













 





 



4- 

 4- 





 











Do. 

 Do. 







E 



Jan. 11 



...do.... 



0.1 



LO 















4- 











Do. 







F 



Jan. 14 



...do .... 



0.1 



LO 















4- 











Do. 







G 



Jan. 16 



...do .... 



0.1 



LO 















4- 











Do. 







A 



Jan. 5 



Jan. 5 



0.1 



LO 



tr 











4- 



4- 







Do. 







B 



...do ( Jan. 16 



0.1 



LO 















4- 



4- 







Do. 







C 



...do |...do 



0.1 



LO 















+ 











Do. 



4561 



...do .... 



D 



Jan. 8 '...do 



0.1 



LO 















4- 



± 







Do. 







E 



Jan. 11 '...do -._- 



0.1 



LO 















4 



4- 







Do. 







F 



Jan. 14 |.— do 



0.1 



LO 















4- 



4- 







Do. 







G 



Jan. 16 ...do 



I 



0.1 



LO 















4- 



4- 







Do. 



Table IX shows that fresh sera heated to 55° C. for thirty 

 minutes before they were mixed with glycerin did not become 

 anticomplementary in eleven days, while fresh sera that were 

 heated after they had been mixed with glycerin were anticom- 

 plementary eleven days later. Two, Nos. 4559 and 4560, of 

 the five sera tested had become permanently anticomplementary 

 in eleven days; the anticomplementary property was but par- 

 tially destroyed by heating portions G to 55° C. for thirty 

 minutes. The sera were sterile. 



