16 



The Philippine Journal of Science 



1916 



TEST 9' 



Anticomplementary serum tested. — Portions C of sera 4557, 

 4558, 4559, 4560, and 4561 were retested. Instead of 1 unit 

 of amboceptor, 1.5 units were used. 



Table IX'. — Moderately anticomplementary sera tested by the 

 Wassermann method. 



No. of 

 serum. 



Date 

 secured. 



Por- 

 tion. 



Date 

 heated. 



Date 



tested. 



Se- 

 rum. 



Am- 

 bo- 

 cep- 

 tor. 



Tube— 



Result. 



1 



2 



3 



1' 



2' 



3' 





1916. 





1916. 



1916. 



cc. 



Unit. 

















4557 



Jan. 4 



C 



Jan. 5 



Jan. 16 



0.1 



1.5 



+ 



± 







+ 



± 







Negative. 



4558 



...do—. 



C 



...do.... 



...do.... 



0.1 



1.5 



+ 



+ 







+ 



+ 







Do. 



4559 



—do .— 



C 



...do.... 



...do.-.. 



0.1 



1.5 



+ 



± 







+ 



± 







Do. 



4560 



...do .... 



C 



— do.— 



...do.... 



0.1 



1.5 



+ 











+ 



+ 







Strongly positive. 



4561 



...do .... 



C 



...do.— 



—do.... 



0.1 



1.5 















+ 



+ 







Do. 



Table IX' shows that moderate anticomplementary property 

 was readily overcome by increasing the quantity of hsemolytic 

 amboceptor. 



TEST 10 



The anticomplementary property of glycerin. — Specimens 

 4595, 4596, 4597, 4598, and 4599 were secured on February 10, 

 1916. On February 11, 1916, the sera were drawn off the clots. 

 Each serum was divided into two portions, A and B. Both por- 

 tions were heated to 55° C. for thirty minutes. Portion A was 

 tested by the Wassermann method without having been mixed 

 with glycerin. Portion B was mixed with an equal volume of 

 sterilized, chemically pure glycerin and was tested by the Was- 

 sermann method. 



Table X.-^The anticomplementary property of glycerin mixed with serum. 















Am- 





Tube- 





No. of 

 serum. 



Date 

 secured. 



Por- 

 tion. 



Date 

 heated. 



Date 

 tested. 



Se- 

 rum. 



bo- 

 cep- 









Result. 



























tor. 



1 



2 



3 



1' 



2' 



3' 







1916. 





1916. 



1916. 



cc. 



Unit. 

















4595 



Feb. 10 



1 ^ 

 1 B 



Feb. 11 



Feb. 11 



0.1 



1.2 



H- 



+ 



+ 



+ 



+ 



± 



Negative. 







...do .... 



...do .... 



0.1 



1.2 



+ 



+ 







+ 



+ 







Do. 



4596 



...do...- 



[ A 



...do.... 



...do-... 



0.1 



1.2 















+ 



+ 



± 



Strongly positive. 







I B 



...do.... 



—do.... 



0.1 



1.2 















-1- 



+ 







Do. 



4597 



...do.... 



J A 



...do .— 



...do.... 



0.1 



1.2 



+ 



+ 



± 



-1- 



+ 



± 



Negative. 







I B 



...do — 



...do .... 



0.1 



1.2 



+ 



4- 







+ 



+ 







Do. 



4598 



—do — . 



A 

 B 



...do.... 



...do-.. 



0.1 



1.2 



+ 











+ 



-1- 



± 



Strongly positive. 







...do.... 



...do.... 



0.1 



1.2 















+ 



+ 







Do. 



4599 



...do.... 



A 

 B 



...do .... 



—do .... 



0.1 



1.2 



H- 



tr 







+ 



H- 



± 



Do. 







...do .... 



...do.... 



0.1 



1.2 



-f- 











+ 



+ 







Do. 



