38 



The Philippine Journal of Science 



1916 



which failed to dissolve the horse corpuscles, was retested 

 against the corpuscles of the same horse and against the cor- 

 puscles of three other horses. 



Table VII. — Serum 48 tested against the corpuscles of four different horse.s. 



Corpuscles of horse — 



Human serum. 



0.4 



0.2 



0.1 



0.05 



1 







+ 

 + 

 + 







tr 



± 

 ± 





 

 

 





 

 

 



2.- 



3 



4 





Table VII shows that the corpuscles of horse 1 were unusually 

 resistant toward haemolysis. While 0.4 cubic centimeter of 

 serum 48 did not produce so much as a trace of haemolysis with 

 corpuscles of horse 1, the dose of 0.2 cubic centimeter almost 

 completely dissolved the test dose of corpuscles from horses 



3 and 4. 



HEMOLYSIS BY REACTIVATED, HEATED HUMAN SERUM 



Technique. — The sera were heated to between 55 °C. and 56° C. 

 for thirty minutes, and the heated sera were used in quantities 

 of 0.4, 0.2, and 0.1 cubic centimeter. As complement the pooled 

 sera of five guinea pigs were used in a dose of 0.5 cubic centi- 

 meter of a 10 per cent dilution in physiologic salt solution. The 

 natural haemolytic amboceptor was not removed from the com- 

 plement serum; it was tested for, and none was found in the 

 test dose for the corpuscles of the sheep, the goat, and the 

 rabbit. With horse corpuscles guinea pig serum produced a 

 trace of haemolysis. The washed corpuscles were used in dose 

 of 0.5 cubic centimeter of a 4 per cent suspension in physiologic 

 salt solution. Three test tubes were used for each test. In 

 tube 1 were put 0.5 cubic centimeter of diluted, heated human 

 serum representing 0.4 cubic centimeter serum and 0.1 cubic 

 centimeter of physiologic salt solution, 0.5 cubic centimeter of 10 

 per cent dilution of guinea pig serum, 0.5 cubic centimeter of 



4 per cent suspension of washed corpuscles, and 1 cubic centi- 

 meter of physiologic salt solution to make the total quantity 

 in the tube 2.5 cubic centimeters. Tube 2 received 0.5 cubic 

 centimeter of diluted serum representing 0.2 cubic centimeter 

 of serum and 0.3 cubic centimeter of physiologic salt solution. 

 Complement, corpuscles suspension, and physiologic salt solu- 

 tion were added as in tube 1. 



