XI, B, 1 



Ruediger: Haemolysis by Human Serum 



39 



Tube 3 received 0.5 cubic centimeter of diluted serum repre- 

 senting 0.1 cubic centimeter of serum and 0.4 cubic centimeter 

 of physiologic salt solution. Complement, corpuscles, and salt 

 solution were added as in the foregoing tubes. Each tube was 

 shaken and placed in the incubator at about 37 °C. for one 

 hour, during which time the tubes were shaken at intervals 

 of about fifteen minutes. After one hour in the incubator the 

 tubes were removed to the refrigerator at about 7°C., and the 

 results were read on the following morning. 



Hemolysis of sheep corpuscles. — Table VIII shows that re- 

 activated, heated human serum readily dissolved the corpuscles 

 of the sheep. All of the fifty sera examined produced some 

 haemolysis when used in a dose of 0.2 cubic centimeter. Of 

 one serum 0.4 cubic centimeter failed to produce lysis, and of 

 one serum the dose of 0.1 cubic centimeter remained inactive. 



Table VIII. — The effect of reactivated, heated human serum on 

 sheep corpuscles. 



Serum. 



100 per 

 cent has- 

 tmolysis. 



50 to 100 

 per cent 

 haemo- 

 lysis. 



Less than 



50 per 

 cent hae- 

 molysis. 



No hae- 

 molysis. 



cc. 

 0.4 

 0.2 

 0.1 



39 



40 

 40 



4 



7 

 4 



6 

 3 

 5 



Per cent. 

 2 





 2 



Haemolysis of goat corpuscles. — Table IX shows that reac- 

 tivated human serum dissolved goat corpuscles almost as readily 

 as it dissolved the corpuscles of the sheep. 



Table IX. — The effect of reactivated, heated human serum on 



goat corpuscles. 



Serum. 



100 per 

 cent hae- 

 molysis. 



■ 



50 to 100 Less than 



per cent ' 50 per 



haemo- cent hae- 



lysis. molysis. 



No hae- 

 molysis. 



cc. 

 0.4 

 0.2 

 0.1 



30 

 31 



28 



13 

 13 

 13 



6 

 6 



8 



Per cent. 

 2 



2 

 2 



Haemolysis of horse corpuscles. — The amount of haemolysis 

 obtained with horse corpuscles was much less than that obtained 

 with the corpuscles of the sheep or of the goat. As is shown 

 in Table X, the dose of 0.2 cubic centimeter of one serum com- 

 pletely dissolved the test dose of corpuscles, and of one serum 

 0.1 cubic centimeter completely dissolved the corpuscles. Al- 



