42 The Philippine Journal of Science i9i6 



Table XIII. — Hsemolysis by reactivated human serum — Continued. 



Serum No. 



Corpuscles of — 



Sheep. 



Goat. 



Horse. 



Rabbit. 



Guinea pig. 



0.4 



0.2 



0.1 



0.4 



0.2 



0.1 



0.4 



0.2 



0.1 



0.4 



0.2 



0.1 



0.4 



0.2 



0.1 



33 



+ 



+ 

 + 

 + 

 + 

 + 

 + 

 -1- 

 + 

 + 

 + 

 + 

 + 

 

 + 

 + 

 tr 



+ 

 + 

 + 

 + 

 + 

 ± 



+ 

 -h 

 + 

 + 

 + 

 + 

 + 

 + 

 tr 

 + 

 + 

 tr 



+ 



4- 

 + 

 + 

 + 



tr 



+ 

 + 

 -1- 

 + 

 + 

 + 

 + 

 + 

 tr 

 + 

 + 

 tr 



+ 

 + 

 + 



± 

 

 + 

 tr 

 ± 

 ± 

 + 

 ± 

 tr 

 + 

 tr 

 + 

 ± 

 tr 



+ 

 + 

 + 

 + 

 tr 

 

 + 

 tr 

 ± 

 ± 

 + 

 ± 

 tr 

 + 

 tr 

 ± 

 ± 



tr 



+ 

 + 

 + 

 ± 

 tr 

 

 + 

 tr 

 ± 

 ± 

 + 

 ± 

 tr 

 + 

 tr 

 tr 

 tr 

 tr 



tr 

 tr 

 tr 

 tr 

 tr 

 ± 

 ± 

 tr 

 tr 

 tr 



4; 



tr 

 tr 

 tr 

 tr 

 tr 

 tr 

 tr 



tr 

 tr 

 tr 

 tr 

 tr 

 tr 

 ± 

 tr 

 tr 

 tr 

 + 

 tr 

 tr 

 tr 

 tr 

 tr 

 tr 

 tr 



tr 

 tr 

 tr 

 tr 

 tr 

 tr 

 ± 

 tr 

 tr 

 tr 

 ± 



tr 

 tr 

 tr 

 tr 

 tr 

 tr 

 tr 





 

 tr 

 

 

 tr 

 

 tr 

 

 tr 

 tr 

 

 

 

 

 

 

 





 

 tr 

 

 

 tr 

 

 tr 

 

 tr 

 tr 

 

 

 

 

 

 

 





 tr 

 

 

 tr 

 

 tr 

 

 tr 

 tr 

 

 

 

 

 

 

 



Otr 

 

 tr 

 

 

 

 

 

 

 

 

 

 

 tr 

 

 

 tr 

 





 

 tr 

 

 

 

 

 

 

 

 

 

 

 tr 

 

 

 

 





 





 

 

 

 

 



d 





 

 

 

 

 

 

 

 



34 



35 



36 



37 



38 



39 



40 



41 



42 



43 



44 



46 



46-. - 



47 _ 



48 



49... 



50 





+ = complete haemolysis (100 per cent); + = haemolysis between 50 and 100 per cent; 

 tr = hsemolysis less than 60 per cent; = no haemolysis. 



Reactivation of natural antihorse amboceptor. — A quantity 

 of fresh human serum was divided into three portions desig- 

 nated as A, B, and C. Portion A was left unheated, portion B 

 was heated to about 55° C. for thirty minutes, and from portion 

 C the natural antihorse amboceptor was removed. Portion C 

 was not heated. The amboceptor was removed in the following 

 manner: One cubic centimeter of unheated serum was diluted 

 with 1 cubic centimeter of physiologic salt solution and was 

 placed in crushed ice. A centrifuge tube with 2 cubic centi- 

 meters of washed horse corpuscles was also placed in the crushed 

 ice. Having stood in the crushed ice for fifteen minutes, the 

 diluted serum was poured into the centrifuge tube with the 

 corpuscles; serum and corpuscles were well mixed and were 

 left in the crushed ice. Four centrifuge tubes, each containing 

 2 cubic centimeters of washed horse corpuscles and 1 cubic centi- 

 meter of human serum, were prepared. Two hours after mix- 

 ing the corpuscles with the serum one tube was centrifuged and 

 the serum was pipetted off and tested for haemolytic properties. 

 It still contained most of the antihorse amboceptor. At the 



