44 The Philippine Journal of Science isis 



Table XIV. — Natural antihorse amboceptor reactivated. 



Set- 



Serum. 



0.4 



0.2 



0.1 



1 



+ 

 + 



+ 

 -r 

 ± 



± 

 tr 

 



2 . 



8 . 





Table XIV shows that this human serum was readily reac- 

 tivated with human complement; almost the entire hsemolytic 

 power was restored. Guinea pig complement did not reactivate 

 the serum well. 



Reactivation of the antirabbit amboceptor in fresh human se- 

 rum. — A quantity of human serum was divided into three por- 

 tions — A, B, and C — and was tested for hsemolytic power 

 within five hours after the bleeding. Portion A was not heated ; 

 it was tested for the original hasmolytic power. Portions 

 B and C were heated to about 55° C. for thirty minutes and 

 were reactivated with fresh guinea pig serum. The guinea pig 

 serum, also, was tested for hsemolysin for rabbit corpuscles. 



Technique. — For the human serum three sets of tubes, A, B, 

 and C, were prepared. Each set contained three tubes desig- 

 nated as 1, 2, and 3. Tubes 1, 2, and 3 in set A received 0.4 

 cubic centimeter, 0.2 cubic centimeter, and 0.1 cubic centimeter 

 of unheated human serum, respectively. To each tube 0.5 cubic 

 centimeter of 4 per cent suspension of rabbit corpuscles was 

 added, and the total quantity in each tube was brought up to 2.5 

 cubic centimeters with physiologic salt solution. In set B tube 

 1 received 0.4 cubic centimeter of heated human serum and 

 0.4 cubic centimeter of guinea pig serum. Tube 2 received 0.2 

 cubic centimeter of heated human serum and 0.2 cubic centi- 

 meter of guinea pig serum, while tube 3 received 0.1 cubic 

 centimeter of heated human serum and 0.1 cubic centimeter of 

 guinea pig serum. To each tube was added 0.5 cubic centimeter 

 of 4 per cent suspension of rabbit corpuscles and enough phy- 

 siologic salt solution to bring the total quantity up to 2.5 cubic 

 centimeters. 



Tubes 1, 2, and 3 in set C received 0.4 cubic centimeter, 

 0.2 cubic centimeter, and 0.1 cubic centimeter of heated human 

 serum and 0.2 cubic centimeter, 0.1 cubic centimeter, and 0.05 

 cubic centimeter of guinea pig serum, respectively. Each tube 

 received 0.5 cubic centimeter of 4 per cent suspension of rabbit 

 corpuscles and enough salt solution to bring the total quantity 



