XI, B, 1 



Ruediger: Haemolysis by Human Serum 



45 



up to 2.5 cubic centimeters. All tubes were placed in the in- 

 cubator at 37 °C. for one hour and were then removed to room 

 temperature. The results were read and recorded about three 

 hours after the corpuscles had been added. 



Table XV. — Natural antirabbit amboceptor reactivated with guinea pig 



complement. 



Set- 



Serum. 



0.4 



0.2 



0.1 



A- — - 



+ 

 + 

 + 



+ 

 + 

 + 







+ 



± 



B - - 



C 





Table XV shows that the natural antirabbit amboceptor can 

 be reactivated with guinea pig complement provided a suf- 

 ficiently large dose is used. Equal parts of heated human se- 

 rum and fresh guinea pig serum had more than double the 

 haemolytic power of the unheated human serum. Heated human 

 serum mixed with half its volume of fresh guinea pig serum 

 had nearly twice the hsemolytic power of the whole human 

 serum. 



Antirabbit hsemolysin in guinea pig serum. — Fresh guinea 

 pig serum was tested in doses of 0.8, 0.4, 0.2, and 0.1 cubic 

 centimeter against 0.5 cubic centimeter of 4 per cent suspension 

 of rabbit corpuscles. The total quantity in each was made 

 up to 2.5 cubic centimeters with physiologic salt solution, and 

 the results were read about three hours after the corpuscles 

 had been added. 



TabIoG XVI. — Complement control to Table XV. Antirabbit hsemolysis in 



guinea pig serum. 



Guinea pig serum. 

 cc. 

 0.8 



0.4 



0.2 



0.1 



Result. 



-±^ 



tr 

 

 



As Table XVI shows, this guinea pig serum had slight hsemo- 

 lytic power for rabbit corpuscles. 



Reactivation of natural antiguinea pig amboceptor. — In pre- 

 liminary test it was found that the natural antiguinea pig am- 

 boceptor was not entirely absorbed from human serum in two 

 hours; three hours sufficed, while five hours were too long. 

 Human serum that had been in contact with guinea pig cor- 



