XI, B, 1 Ruediger: Haemolysis by Human Serum 47 



Table XVII. — Reactivation of natural antiguinea pig amboceptor. 



Set— 



Human serum. 



0.4 



0.2 



0.1 



0.05 



A - - 



+ 

 + 

 



+ 

 



+ 







± 

 tr 

 



B 



B' 





Table XVII shows the results obtained. The unheated human 

 serum had good hsemolytic power for guinea pig corpuscles; 

 complete hsemolysis occurred in tubes 1, 2, and 3 and more 

 than 50 per cent haemolysis in tube 4. Human complement 

 added to heated human serum almost completely restored the 

 original hsemolytic power. Guinea pig complement did not re- 

 activate the natural antiguinea pig amboceptor. 



The effect of absorption of one amboceptor on another ambo- 

 ceptor. — After having removed the natural antihorse ambocep- 

 tor, the serum was tested for haemolytic power against guinea 

 pig corpuscles. Two sets of tubes, A and B, were used. Each 

 set contained four tubes marked 1, 2, 3, and 4. In set A was 

 tested the hsemolytic power of the whole human serum in quan- 

 tities of 0.4, 0.2, 0.1, and 0.05 cubic centimeter. Each tube 

 received 0.5 cubic centimeter of 4 per cent suspension of guinea 

 pig corpuscles and enough physiologic salt solution to make the 

 total quantity 2.5 cubic centimeters. In set B the human serum, 

 freed from natural antihorse amboceptor, was titrated against 

 guinea pig corpuscles. All tubes were shaken, placed in the 

 incubator at 37 °C. for one hour, and removed to room temper- 

 ature ; the results were read about three hours after the corpus- 

 cles had been added. 



Table XVIII. — Human serum, freed from antihorse amboceptor titrated 

 against guinea pig corpuscles. 





Serum. 



0.4 



0.2 



0.1 



1 



0.05 



Whole human serum 



+ 



-T- 



+ 



-f 

 + 



± 

 ± 



Human serum freed from antihorse amboceptor 





Table XVIII shows that removing the antihorse amboceptor 

 did not appreciably disturb the antiguinea pig amboceptor. 



Antihorse amboceptor in serum freed from antiguinea pig 

 amboceptor. — After having removed the natural antiguinea pig 



