48 



The Philippine Journal of Science 



1916 



amboceptor, the serum was tested for haemolytic power against 

 the corpuscles of the horse. Two sets of tubes, designated as 

 A and B, were used. Each set contained four tubes marked 1, 

 2, 3, and 4, respectively. In set A the whole human serum was 

 titrated. Tubes 1, 2, 3, and 4 received 0.4, 0.2, 0.1, and 0.05 

 cubic centimeter of serum, respectively. To each tube was 

 added 0.5 cubic centimeter of 4 per cent suspension of horse 

 corpuscles and enough physiologic salt solution to bring the 

 total quantity up to 2.5 cubic centimeters. In set B the serum 

 freed from antiguinea pig amboceptor was tested for haemolytic 

 power against horse corpuscles. The serum was used in quan- 

 tities of 0.4, 0.2, 0.1, and 0.05 cubic centimeter, and horse cor- 

 puscles and salt solution were added as in set A. All tubes were 

 shaken, placed in the incubator at 37° C. for one hour, and re- 

 moved to room temperature; the results were read about three 

 hours after the corpuscles had been added. 



Table XIX. — Antihorse amboceptor in serum freed from antiguinea pig 



amboceptor. 



Set- 



Serum. 



0.4 



0.2 



0.1 



0.05 



A - - - . — — - 



+ 

 + 



+ 

 + 



tr 









 



B 





Table XIX shows the results obtained. Removing the natural 

 antiguinea pig amboceptor left the natural antihorse ambocep- 

 tor practically undisturbed. 



CONCLUSION 



Unheated, fresh human serum dissolves the red blood cor- 

 puscles of the sheep better than it dissolves those of the guinea 

 pig, goat, horse, or rabbit. 



For guinea pig corpuscles the haemolytic power of fresh, un- 

 heated human serum is slightly higher than for goat corpuscles. 



The haemolytic power of fresh, unheated human serum is 

 lower for the corpuscles of the rabbit than for the corpuscles 

 of sheep, goat, horse, or guinea pig. 



A fresh, unheated human serum may dissolve the corpuscles 

 of one horse and be inactive against the corpuscles of another 

 horse. The corpuscles differ in resistance toward haemolysins. 



The natural antisheep and antigoat amboceptors can readily 

 be reactivated with guinea pig complement. 



