88 The Philippine Journal of Science isis 



applied to the smallest quantity of hsemolytic serum which 

 with 0.05 cubic centimeter of complement dissolved the test dose 

 of sensitized corpuscles in one hour. The mixture of hsemolytic 

 amboceptor and corpuscles was allowed to stand at room tem- 

 perature for thirty minutes before the complement was added. 

 After the complement had been added, the tubes were placed 

 in the incubator at 37° C. for one hour. 



Corpuscles. — Human corpuscles from nonsyphilitic persons 

 were well washed and were used in doses of 0.5 cubic centimeter 

 of a 4 per cent suspension in physiologic salt solution. The 

 corpuscles were sensitized for thirty minutes before they were 

 added to the serum-complement-antigen mixture. 



Technique. — The technique used in this investigation was 

 identical with that described in the previous report. Before 

 testing, the human sera were heated to about 55.5°C. (the 

 temperature varied from 55.2°C. to 55.7°C.) for thirty minutes. 

 Six-tenths cubic centimeter of the inactivated serum or 1.2 cubic 

 centimeters of equal parts of human serum and chemically pure 

 glycerin were diluted to 3 cubic centimeters with physiologic salt 

 solution. Six test tubes, 1, 2, and 3 as antigen tubes and 1', 2', 

 and 3' as control tubes, were used in each test. Each tube 

 received 0.5 cubic centimeter of diluted serum. I preferred to 

 use the constant quantity of serum in order to have the anti- 

 complementary property uniform in all tubes. Each of the first 

 pair of tubes, tubes 1 and 1', received 0.5 cubic centimeter of 

 1 : 5 dilution of complement serum ; each tube of the second 

 pair, tubes 2 and 2', received 0.5 cubic centimeter of 1 : 10 dilution 

 of complement serum ; and each of the third pair of tubes, tubes 

 3 and 3', received 0.5 cubic centimeter of 1 : 20 dilution of 

 complement serum. Each of the antigen tubes, tubes 1, 2, and 3, 

 received 0.5 cubic centimeter of 1 : 30 dilution of alcoholic extract 

 (antigen) ; and the control tubes, tubes 1', 2', and 3', received 

 0.5 cubic centimeter of physiologic salt solution each. These 

 mixtures were placed in the incubator at 37° C. for one hour. 

 After having been in the incubator one hour, each tube received 

 1 cubic centimeter of sensitized corpuscles, representing 0.5 

 cubic centimeter of 4 per cent suspension of washed corpuscles 

 and 1 unit of amboceptor diluted to 0.5 cubic centimeter with 

 physiologic salt solution. After shaking, the tubes were placed 

 in the incubator at 37 °C. for one hour; during this hour and 

 during the thirty minutes while the corpuscles were being sen- 

 sitized, the mixtures were repeatedly shaken to prevent the 



