XI, B, 2 Ruediger: Wassermann Reaction 89 



corpuscles from settling to the bottom of the container. After 

 having been in the incubator for one hour, the tubes were al- 

 lowed to stand at room temperature for two hours, after which 

 the first reading was taken. After the first reading the tubes 

 were put into the refrigerator, and the final results were read 

 on the following morning. 



Antigen control. — Six test tubes were used as in conducting 

 the test, but the human serum was omitted, and the volume 

 was made up with physiologic salt solution. If there was no 

 anticomplementary action in the antigen control, the dose of 

 antigen was considered suitable. If there was anticomplemen- 

 tary action, the dose of antigen was decreased until there was no 

 anticomplementary action. 



TEST 1 



Specimens 4424, 4425, 4426, 4427, and 4428 were secured No- 

 vember 23, 1915. The sera were drawn off the clots the next day, 

 and each serum was divided into two portions, A and B. Ungly- 

 cerinated, portion A was tested November 24. Portion B, un- 

 heated, was mixed with an equal volume of sterilized, chemically 

 pure glycerin and was kept at room temperature in a cork- 

 stoppered test tube. 



Specimens 4429, 4430, 4432, and 4433 were secured November 

 24, 1915. The sera were drawn off the clots the next day, and 

 each serum was divided into two portions, A and B. Portion A, 

 unglycerinated, was tested November 25. Unheated, portion B 

 was mixed with an equal volume of sterilized, chemically pure 

 glycerin and was kept at room temperature in a cork-stoppered 

 test tube. 



Specimen 4434 was secured November 26, 1915. The serum 

 was drawn off the clot the next day and was divided into two 

 portions, A and B. Unglycerinated, portion A was tested 

 November 27. Unheated, portion B was mixed with an equal 

 volume of sterilized, chemically pure glycerin and was kept at 

 room temperature in a cork-stoppered test tube. 



Portion B of each of the above sera was tested December 26, 

 1915, January 30, 1916, and February 22, 1916. Immediately 

 before testing, the glycerinated serum (1.2 cubic centimeters) 

 necessary for the test was heated to about 55.5° C. for thirty 

 minutes. 



All sera were tested bacteriologically. 



