92 The Philippine Journal of Science i9i6 



Table I shows that with these ten sera, namely, Nos. 4424, 

 4425, 4426, 4427, 4428, 4429, 4430, 4432, 4433, and 4434, gly- 

 cerin did not influence the Wassermann reaction. Sera 4430 and 

 4433 gave weakly positive results at the first test and moderately 

 positive results at subsequent tests. With the other sera sub- 

 sequent tests gave results practically identical with the results 

 of the first tests. After having been kept at room temperature 

 for three months, these sera were bacteriologically sterile, and 

 after having been heated to about 55.5° C. for thirty minutes, 

 they were but slightly anticomplementary. There was little 

 or no difference between the two readings. 



TEST 2 



Specimens 4435, 4436, 4437, 4438, and 4439 were secured 

 November 26, 1915. The next day the sera were drawn off the 

 clots, and each serum was divided into two portions, A and B. 

 Without having been mixed with glycerin, portion A of each 

 serum was tested November 27. Portion B of each serum was 

 heated to about 55.5°C. for thirty minutes November 27, was 

 mixed with an equal volume of sterilized, chemically pure gly- 

 cerin, and was kept at room temperature in a cork-stoppered 

 test tube to be tested later. 



Specimens 4440, 4441, 4442, 4443, and 4444 were secured 

 November 27. The sera were drawn off the clots the next day. 

 Each serum was divided into two portions, A and B. Ungly- 

 cerinated, portion A was tested November 28. On the same date 

 portion B of each serum was heated to about 55.5 °C. for thirty 

 minutes, was mixed with an equal volume of sterilized, chemi- 

 cally pure glycerin, and was kept at room temperature in a 

 cork-stoppered test tube to be tested later. 



Without having been reheated, portion B of each serum was 

 tested December 27, 1915, January 30, 1916, and February 27, 

 1916. 



February 27, 1916, each serum was tested bacteriologically. 



Table II shows that with the ten sera used in test 2 the gly- 

 cerin did not noticeably influence the Wassermann reaction. 

 After having been kept at room temperature for three months, 

 the results obtained with the test were practically identical with 

 the results obtained before the sera had been mixed with gly- 

 cerin. The sera had not become anticomplementary and were 

 free from bacterial growth. There was little or no difference 

 between the reading taken three hours after the corpuscles had 

 been added and that taken eighteen hours after the corpuscles 

 had been added. 



