XI, B, 2 Ruediger: Wassermann Reaction 95 



TEST 3 



November 30, 1915, specimens 4446, 4447, 4448, 4449, and 4450 

 were secured. The sera were drawn off the clots the next day. 

 Each serum was divided into two portions, A and B. Ungly- 

 cerinated, portion A was tested December 1. Portion B was 

 heated to 55.5°C. for thirty minutes, was mixed with an equal 

 volume of sterilized, chemically pure glycerin, was placed in 

 the cold storage at about 7°C. in a cork-stoppered test tube, 

 and was tested at intervals of about a month. About three 

 months after it was mixed with glycerin it was examined 

 bacteriologically. 



Table III shows that with sera 4446, 4447, 4448, 4449, and 

 4450 glycerin did not influence the Wassermann reaction during 

 the period of three months. The results obtained at the end 

 of one month, at the end of two months, and at the end of 

 three months were practically identical with the results obtained 

 before glycerin had been added to the sera. The sera having 

 been heated on December 1, 1915, before they had been mixed 

 with glycerin, did not become anticomplementary in three 

 months, and all remained free from bacterial growth. 



TEST 4 



Specimens 4452, 4453, 4454, 4455, and 4456 were secured 

 December 1, 1915. The next day the sera were drawn off the 

 clots. Each serum was divided into two portions, A and B. 

 Unglycerinated, portion A was tested December 2. Portion B 

 of each serum was mixed with an equal volume of sterilized, 

 chemically pure glycerin, was placed in the cold storage at a 

 temperature of about 7°C., and was tested at intervals of about 

 a month. The necessary quantity of serum was heated imme- 

 diately before the test. 



On March 4, 1916, a bacteriologic test was made of each 

 serum. 



141844 3 



