98 "^he Philippine Journal of Science i»i« 



Table IV shows that with sera 4452, 4453, 4454, 4455, and 

 4456 the glycerin did not noticeably influence the Wassermann 

 reaction. All of these sera became strongly anticomplementary, 

 far more so than did the sera that were kept at room temper- 

 ature. All sera remained free from bacterial growth. 



TEST 5 



Specimens 4535, 4536, 4537, 4538, and 4539 were secured De- 

 cember 27, 1915. The sera were drawn off the clots the next 

 day. Each serum was divided into two portions, A and B. Un- 

 glycerinated, portion A was tested on December 28. Portion 

 B of each serum was mixed with an equal volume of sterilized, 

 chemically pure glycerin, was kept at room temperature in a 

 cork-stoppered test tube, and was tested at intervals of a month. 

 The necessary quantity of serum was heated to 55.5° C. 

 immediately before testing. A bacteriologic test was made of 

 each serum on March 18, 1916. 



Table V shows that with sera 4535, 4536, 4537, 4538, and 

 4539 the glycerin did not noticeably influence the Wassermann 

 reaction. Although the sera had not been heated, they did not 

 become very strongly anticomplementary in about three months. 

 The eratic results obtained on February 13, 1916, are not easily 

 explained. All sera remained clear and free from bacterial 

 growth. 



TEST 6 



Specimens 4540, 4541, 4542, 4543, and 4544 were secured on 

 December 27, 1915. December 28 the sera were drawn off the 

 clots. Each serum was divided into two portions, A and B. 

 Unglycerinated, portion A was tested December 28. Portion B 

 was heated to about 55.5° C. for thirty minutes, was mixed with 

 an equal volume of sterilized, chemically pure glycerin, was kept 

 at room temperature in a cork-stoppered test tube, and was 

 tested at intervals of about a month. On March 18, 1916, each 

 serum was tested bacteriologically. 



