XI. B. 2 Ruediger : Wassermann Reaction 101 



Table VI shows the results obtained with sera 4540, 4541, 

 4542, 4543, and 4544. The glycerin did not noticeably influence 

 the Wassermann reaction. The sera did not become anticom- 

 plementary. They remained clear and free from bacterial 

 growth. 



TEST 7 



On December 29, 1915, specimens 4546, 4547, 4548, 4549, and 

 4550 were secured. The sera were drawn off the clots December 

 30. Each serum was divided into two portions, A and B. Un- 

 glycerinated, portion A of each serum was tested December 30. 

 Unheated, portion B of each serum was mixed with an equal 

 volume of sterilized, chemically pure glycerin, was kept in a 

 cork-stoppered test tube in the cold storage at a temperature 

 of about 7°C., and was tested at intervals of about a month. 

 The necessary quantity of serum was heated to 55.5°C. for 

 thirty minutes immediately before testing. 



Table VII shows that with sera 4546, 4547, 4548, 4549, and 

 4550 the glycerin did not influence the Wassermann reaction. 

 The sera did not become very strongly anticomplementary in 

 about three months, and all sera remained clear and free from 

 bacterial growth. There was practically no difference between 

 the first and second readings. 



TEST 8 



Specimens 4551, 4552, 4553, 4554, and 4555 were secured 

 December 29, 1915. The sera were drawn off the clots the next 

 day. Each serum was divided into two portions, A and B. 

 Unglycerinated, portion A of each serum was tested December 

 30. Portion B of each serum was heated to about 55.5°C. for 

 thirty minutes, was mixed with an equal volume of sterilized, 

 chemically pure glycerin, was kept in a cork-stoppered test 

 tube in the cold storage at a temperature of about 7°C., and, 

 without reheating, was tested at intervals of about a month. 

 On March 25, 1916, each serum was tested for bacterial 

 growth. 



