124 



The Philippine Journal of Science 



1916 



EXPERIMENTS WITH DOGS 



Experimental deficiency neuritis has been produced in dogs.-* 

 Accordingly we have made observations on five dogs of the same 

 litter, three of which received milk autoclaved for two hours 

 at 125° C and two of which received the fresh milk. As in the 

 experiments with fowls, daily morning deliveries of fresh cows' 

 milk were obtained from a local dairy. The dogs were kept in 

 a large pen outdoors. They were fed individually, and a record 

 was kept of the amount eaten by each animal. The dogs were 

 17 days old when the experiment was started and weighed 

 between 593 and 725 grams. The results of the experiment are 

 given in Table III. 



Table III. — Dogs fed on fresh and autoclaved milk. 



Bog. 



Milk. 



Neuritis.^ 



Gain on 

 47th day. 



Remarks. 



No. 



Sex. 



1 



2 

 3 



4 



5 



Female 



do 



Fresh 



Positive 



do 



Grains. 

 1,197 



1,592 

 1,370 

 1,222 



1,345 



Paralysis of posterior limbs, fifty- 

 fifth to fifty-ninth day; pro- 

 nounced oedema of limbs, eighty- 

 ninth day until killed on the 

 ninety-second day. Total gain 

 in weight, 2,860 grams. 



Killed, forty-eighth day. 

 Do. 



Marked oedema of limbs, ninetieth 

 day; killed, ninety-second day. 

 Total gain in weight, 2,145 

 grams. 



Killed, forty-eighth day. 



do 



Male 



Autoclaved 



do 



do 



do 



do- 



Female 



do 



do 









° As evidenced by histological examination (Marchi) of the sciatic nerve. 



On the forty-seventh day of the experiment all the dogs were 

 in excellent condition. They were slightly undersized as com- 

 pared with another dog of the same litter which had been allowed 

 to suckle the bitch, but at this time was also being given mixed 

 food. Three dogs (Nos. 2, 3, and 4) were chloroformed on the 

 forty-eighth day. The experiment was continued with dogs 

 1 and 6. Toward the conclusion of the experiment it became 

 difficult to persuade the two dogs to eat, although fair 

 amounts of milk were taken until the onset of oedema on the 

 eighty-ninth day of the experiment. At this time the dogs were 

 somewhat thin, undersized, and partially aphonic and refused 



Cf. Schaumann, loc. cit. 



