126 



The Philippine Journal of Science 



1916 



with either fresh or autoclaved milk induced degenerative 

 changes in the peripheral nerves of three dogs at the end of 

 forty-seven days. Degeneration of the peripheral nerves, oede- 

 ma, and partial aphonia were observed subsequently in the one 

 fresh-milk dog and the one autoclaved-milk dog remaining. 

 Little difference could be observed between the effect of the diet 

 on the two dogs given fresh milk and the three receiving auto- 

 claved milk. No scorbutic symptoms were observed. 



EXPERIMENTS WITH PIGS 



Six 17-day old pigs of the native breed were selected from the 

 same litter and three were fed exclusively on fresh cows' milk 

 and three on autoclaved milk. The pigs were kept in separate 

 pens. One fresh-milk pig and one autoclaved-milk pig were 

 killed after thirty-four days; a similar pair were killed after 

 sixty days. Of the remaining pigs, the autoclaved-milk pig 

 died on the seventy-fourth day of the experiment, and the fresh- 

 milk pig on the one hundred sixth day. As in the earlier obser- 

 vations on dogs, the milk eaten was recorded daily and the body 

 weights were recorded every four days. 



The results are given in Table IV. 



Table IV. — Pigs fed on fresh and autoclaved milk. 



Pig. 



Milk. 



Neuritis. « 



Weight. 



Remarks. 











No. 



Sex. 







Initial. 



Gain. 













Grams. 



Grams. 





1 



Female 



Freeh.. 



Positive 



820 



3,730 



Killed, thirty-fourth day. 



2 



do 



Autoclaved... 



do 



800 



3,700 



Do. 



3 



Male 



Fresh 



do 



776 



6,274 



(Edema of limbs and face from 

 the fifty-sixth day until killed 



















on the sixtieth day. 



4 



do 



Autoclaved... 



do 



824 



7,626 



Do. 



5 



do 



Fresh 



do 



550 



bis, 890 



CEdema of limbs and face from 

 about the fifty-sixth day and 



















gradually increasing apho- 















nia. Died on the one hundred 















sixth day from ruptured coro- 















nary artery. 



6 



Male 



Autoclaved 



do...- 



550 



10.265 



CEdema of limbs and face from 

 about the fifty-sixth day and 



















gradually increasing aphonia. 















Died on the seventy-fourth 















day from ruptured coronary 















artery. 



» As evidenced by histological examination (Marchi) of the sciatic and pneumogastric 

 nerves. 



■^ This pig had increased in weight by 10,150 grams on the day on which the last figure 

 for the companion pig (No. 6) was obtained. 



