154 



The Philippine Journal of Science 



1916 



Table I. — Showing the number of takes in experimental cholera carriers af- 

 ter intravesicular inoculation of decreasing doses of cholera culture. 



[Animals killed seven days after inoculation.] 



Dilution. 



Direct plates. 



Peptone cultures. 



Gall 

 bladder. 



Duode- 

 num. 



Ileum. 



Caecum. 



Gall 

 bladder. 



Duode- 

 num. 



Ileum. 



Csecum. 



1/80 



n 

 vn 

 vn 

 vn 



n 



n 

 vf 

 vf 



n 

 vf 



n 



vn 



f 



f 



vf 



f 



vf 



vf 



vf 



f 



n 



n 



vf 

 vf 



n 



f 



7f 

 f 



vn 



vn 

 f 



f 



+ 



+ 



+ 

 + 

 + 

 + 

 + 

 i- 

 + 

 + 



+ 



+ 

 + 

 + 

 + 

 + 

 + 

 + 

 + 



+ 

 + 



+ 

 -1- 



-1- 

 + 



+ 

 + 

 -1- 

 + 

 + 

 + 

 + 

 + 



+ 



+ 



+ 

 + 



-1- 



1/160 



1/320 



1/640 



1/1,000 



1/2,000 



1/4.000 



1/8,000 .- 



1/20,000 



1/40,000 



1/80,000 



1/100,000 



1/10,000,000 



1/100,000,000...- 



-f- = cholera vibrios found; — =cho]era vibrios not found; vf=le8S than half a dozen 

 colonies; f= about one dozen colonies; n = about 200 colonies; vn = more than 200 colonies. 



Table II. — Showing the duration of carrier state in experimental animals 

 after intravesicular injection of decreasing doses of cholera culture. 



[Animals killed thirteen days after inoculation.] 



Dilution. 



V80 



1/160 



1/S20 



1/640 



1/1,000.. 



1/2,000.. 



1/4,000 



1/8,000 



1/20,000 



1/40,000 



1/100,000 



1/100,000,000 . 



Direct plates. 



Gall 



bladder. 



f 

 f 

 vf 



f 

 f 

 n 

 f 

 n 

 vf 



Duode- 

 num. 



vf 



vf 



Ileum. 



f 



Cae- 

 cum. 



Peptone cultures. 



Gall 

 bladder. 



+ 



+ 

 + 

 + 

 + 

 + 

 + 

 + 

 + 



Duode- 

 num. 



+ 



+ 



+ 



-I- 

 + 

 + 

 + 



Ileum. 



+ 

 + 

 + 

 + 

 ■f 

 + 

 + 

 + 

 -I- 



Csa- 

 cum. 



* Vibrios present, but overgrown by other bacteria. 



Table II shows the duration of carrier state in animals inocu- 

 lated with decreasing amounts of cholera culture. All of the 

 carriers that were infected with from 1/80 to 1/20,000 of a 

 slant lasted the usual length of time, which was found in our 



