THE INFLUENCE OF BILE UPON THE DURATION OF THE STATE 

 OF CHOLERA CARRIERS IN EXPERIMENTAL ANIMALS 



By Otto Schobl 

 (From the Biological Laboratory, Bureau of Science, Manila, P. I.) 



In order to gain some information with regard to the relation 

 of cholagogs to the state of cholera carriers, some experiments 

 were arranged in the following way. 



Thirteen guinea pigs of the same size were inoculated simul- 

 taneously. One tenth of one slant of cholera culture was in- 

 jected into the gall bladder.^ After the inoculation seven of 

 the animals were given 1 cubic centimeter of ox bile by mouth 

 every third day. They were killed and examined at intervals as 

 indicated in Table I. The remainder of the experimental car- 

 riers, six in number, which received no bile, were taken as 

 controls. 



Table I. 



-Showing the influence of bile upon the duration of the state of 

 cholera carriers in experimental animals. 



TREATED ANIMALS. 



Killed after 

 inoculation. 



Direct plates. 



Peptone cultures. 



Bile. 



Duoden- 

 um. 



Ileum. 



Caecum. 



Gall 

 bladder. 



Duoden- 

 um. 



Ileum. 



1 



Caecum. 



Days. 

 16 



n 

 n 

 n 



vn 

 n 

 n 

 n 



t 



vf 



_ 







f 



f 

 f 

 f 

 n 

 n 

 n 

 f 



- 



+ 



+ 

 + 

 + 

 •1- 

 + 



+ 

 + 



+ 

 + 

 + 

 + 

 + 



4. 



+ 



+ 

 + 

 + 



+ 



16 



19 



19 



21 



28. 



36 — 





UNTREATED CONTROLS. 



Days. 



16...- 



16- 



19- -. 



21 



28 



35 



vf 

 f 



+ 

 + 



+ 

 + 



+ 

 + 



+ 



+ 



+ = cholera vibrios found ; — = cholera vibrios not found ; vf = less than half a dozen 

 colonies ; f = about one dozen colonies ; n = about 200 colonies ; vn := more than 200 colonies. 



'For technique, see Journ. Inf. Dis. (1916), 18, 307-314. 



157 



