164 



The Philippine Journal of Science 



1916 



Table I. — Reaction of B. pestis in Hiss's litmus serum-waters containing 



0.5 per cent carbohydrate. 



[In Tables I and VIII the symbols are used as follows (compare with Table X) : — =: no 

 change ; + = faint acidity, not sufficient to be consider a positive reaction ; + := medium red- 

 dened, but still clear and fluid ; 2 + = medium red and fluid, but with slight cloudiness ; 8 + = 

 medium opaque, but fluid ; 4 + = medium coagulated. The figures in parentheses indicate 

 the day of cultivation on which the recorded change was observed.] 



Strain of 

 organism. 



E 



H- 



Dextose. 



4+ (2) . 



4+ (2) . 



4+ (2) . 



4+ (2) . 



4+ (2) . 



4+ (2) . 



4+ (2) . 



4+ (2) . 



4+ (2) . 



Mannite. 



Levulose. 

 Series II. 



+ (1) + (1). 



3+ (3) 3+ (3) . 



4+ (4) 4+ (4) . 



+ (3) Negative. 



3+ (4) 



4+ (6) 



(2) do. 



3+ (3) 



,4+ (4) 



± (1) +(2). 



+ (2) 3+ (3) . 



3+ (3) 4+ (4) . 



4+ (5) 



±(2) + (2).. 



+ (3) 3+ (6). 



3+ (4) 



4+ (7) 



+ (1) +&)- 



3+ (4) 3+ (4) . 



14+ (7) 



f+(l) - +(4).. 



2+ (3) 3+ (6) . 



3+ (4) 



4+ (B) 



+ (1) 2+ (3). 



2+ (3) 4+ (4) . 



3+ (4) 



4+ (5) 



+ (1) + (2). 



|3+ (3) 2+ (4) 



4+ (5) I 



Arabinose. Series II. « 



A. 



2+ (3) 



(8). 



+ (3).. 



2+ (4) . 



+ (3).. 

 2+ (4) . 



±(4) 



+ (4). 



+ (3). 



+ (3)-. 



2+ (4) . 



Negative . 



B. 



± (3) .. 



2+ (4) . 



Negative 



.do . 



-do. 



.do. 



-do. 



.do. 



± (6) 



Negative . 



Litmus milk. 



±. 



Negative. 



Do. 



±. 



±. 



Note. — The following sugars were not fermented in these scries : Maltose, galactose, salicin, 

 glycerin, saccharose, lactose, dextrin, nutrose, dulcite, adonite, rafinnose, sorbite, inosite, tunyg- 

 dalin, and arabinose. 



o Results of successive sets with the same lot of medium. 



LITMUS-SUGAR AGARS 



To control the results obtained with serum waters, a parallel 

 set of cultures was made using litmus-sugar agar slants. In 

 Table II are shown details of reactions in the five media which, 

 of the eighteen used in the primary 0.5 per cent set, were acidified. 

 Later, on account of the results with bouillon titration, a 1 per 



