XI. B. 4 



Wade: Carbohydrate Fermentation 



165 



cent set was cultivateci, using only the weakly or irregularly fer- 

 mented substances — salicin, arabinose, galactose, maltose, and 

 glycerin. The reactions with these are included in the table. 

 The stages of acidification are approximately indicated in a 

 manner which is of use for purposes of comparison. 



Table II. — B. pestis on litmus-sugar agars {incubation at 37° 



days). 



C. for ten 



[In Table II the symbols used are as follows: + = slight reddening of agar beneath the 

 growth ; 2 + = a spreading area of acidification ; 3 -|- = diffusion of acid not quite complete ; 

 4 -f = complete reddening of the entire slant. The figures in parentheses indicate the days 

 after inoculation on which the changes were not«d.] 





Series I. 0.5 per cent. 



Series II. 1 per cent. 



Dex- 

 trose. 



Man- 

 nite. 



Levul- 

 ose. 



Malt- 

 ose. 



Glyc- 

 erin. 



Malt- 

 ose. 



Glyc- 

 erin. 



Arabin- 

 ose. 



Galac- 

 tose. 



Salicin. 



A 



4+ (2) 

 4+ (2) 

 4+ (2) 

 4+ (2) 

 4+ (3) 

 4+ (3) 



4+ (4) 



4+ (3) 

 4 +(2) 



4+ (2) 

 4+ (2) 

 4+ (2) 

 4+ (2) 

 4+ (2) 

 4+ (3) 



4+ (3) 



4+ (3) 

 4+ (2) 



4+ (4) 

 4+ (2) 

 4+ (3) 

 4+ (2) 

 4+ (4) 

 4+ (3) 



4+ (4) 



4 + (3) 

 4+ (3) 



4+ (2) 



(a) 



(a) 



(a) 



4+ (3) 



±(1) 



+ (2) 



2 + (3) 



+ (6) 



-(7) 



4+ (4) 



±(1) 

 2+ (2) 



-(8) 

 4+ (4) 



4 + (5) 



4+ (6) 



4+ (7) 

 4+ (10) 

 3-|-(10) 

 4+ (7) 

 4+ (5) 

 4+ (4) 



4+ (5) 



4+ (6) 

 4+ (4) 



4+ (10) 

 4+ (10) 



4+(3) 

 4+ (3) 

 4 +(3) 

 4+ (3) 

 4+ (5) 

 4+ (5) 



4+ (5) 



4+ (3) 



4 + (4) 



4+ (5) 

 4+ (5) 

 4+ (6) 

 4-1- (5) 

 2-1- (10) 

 2-1- (10) 



l-|-(2) 

 2-1- (3) 

 l-|-(4) 

 -(5) 

 + (7) 

 2+{W) 

 41- (7) 



2-1- (10) 



4-f(7) 

 4-h(5) 

 4-+ (5) 

 4+ (7) 

 4-K5) 

 4-F(5) 



(a) 

 (a) 



B - 



C 



D 



E. 



F 



G 



H 



I 





Note. — Reagents unfermented in the 0.5 per cent series : Arabinose, galactose, salicin, 

 saccharose, amygdalin, dextrin, sorbite, inosite, lactose, raffinose, adonite, dulcite, and nutrose. 

 Arabinose and galactose were fermented in the 1 per cent series. 



" Negative in two tests with this lot of medium. 



In the 0.5 per cent agars dextrose and mannite were as 

 promptly and as completely acidified as in the serum waters. 

 Levulose, however, showed a constant and complete acidification, 

 and maltose was much more markedly changed, though with 

 the three New Orleans human strains there was no reaction. 

 With two of the strains obtained locally (F and H) , there was a 

 partial acidification with subsequent reversion ; in both instances 

 the reddening occurred only after a heavy, primarily nonacid- 

 producing growth had developed, and then was confined to the 



