250 The Philippine Journal of Science loie 



can contract .rinderpest by being drenched with urine from a 

 pig sick with rinderpest. In experiment 34 there is a possibil- 

 ity that the urine was kept too long before being used, as has been 

 demonstrated in previous work. (5) 



CARABAOS EXPOSED TO PIGS SICK WITH RINDERPEST 



The following experiment was designed to furnish informa- 

 tion regarding the possibility of carabaos contracting rinder- 

 pest by being placed in contact with pigs sick with rinderpest. 

 This experiment was conducted in stall No. 2. 



EXPERIMENT 36 



Carabao 57. — Known history prior to the experiment: This 

 animal was a native carabao from Aparri, 8 years old, received 

 at the laboratory and placed in quarantine May 11, 1916, and 

 kept under observation seventy-two days before it was used 

 in this experiment. 



June 17, 1916, carabao 57 was used in an experiment in which 

 an investigation was being conducted by Dr. H. W. Wade, of the 

 Bureau of Science, and myself for the purpose of producing a 

 vaccine for rinderpest by attenuating the virus by means of 

 desiccated virulent blood and tissue pulp. This investigation 

 will be reported on at a later date. 



Carabao 57 gave no reaction to the injections administered. 



July 22, 1916, carabao 57 was exposed to pigs 299 and 301. 



History of pig 299: 



July 17, 1916: Inoculated with 50 cubic centimeters of virulent 



blood from carabao 53. 



July 20: First rise in temperature. 



July 21-23: N. E. 



July 24: E. L. 



July 25-26: D., E. L. 



July 27: Died of rinderpest, presenting typical lesions of that 



disease upon autopsy. 

 History of pig 301: 



July 17, 1916: Inoculated with 50 cubic centimeters of virulent 



rinderpest blood from carabao 53. 

 July 19 : First rise in temperature. 

 July 21-23: N. E. 

 July 24-27: E. L. 

 This animal gradually recovered. 



July 29, carabao 57 presented a rise in temperature, register- 

 ing 39.3° C. in the morning. 

 July 31, D., N. E. 

 August 1-2, D., N. E. 



