270 The Philippine Journal of Science i9i6 



posed of epidermis and newly formed inflammatory tissue. None 

 of the cutaneous gland structures are present. The epidermis 

 is intact, though it is somewhat thin and irregular and, at a few 

 points, as shown in figs. 8 and 9, is invaded by scattered parasites. 

 Throughout the sections vascular channels are very numerous. 

 Here and there, usually close under the epidermis, are areas so 

 vascular and blood-filled as to suggest hemorrhage (fig. 6). In 

 the large, irregular, more or less cavernous spaces, which are 

 usually lined with endothelium and filled with normal erythro- 

 cytes, are numbers of leucocytes and apparent forms of the 

 fungus. The lymphatic channels are numerous and prominent 

 in the deeper layers of the tissue. Everywhere there is evident 

 an extensive leucocytic infiltration, particularly in and about the 

 vascular areas (fig. 7). Here are polymorphonuclear leucocytes 

 and, more numerous, cells of the "round-cell" types. In occa- 

 sional small, active areas are numerous large endothelial leuco- 

 cytes, occurring usually within blood channels, as in figs. 7 and 

 10. Most of them, actively phagocytic, contain numbers of para- 

 site bodies. Many of the parasitic cells, even after prolonged 

 study of the cytology of the lesions, are impossible of diflFerentia- 

 tion from body cells. 



In the less active foci are not a few eosinophiles, which have 

 always been observed in the smears from this type of lesion. 

 There is no evidence of pigment deposit in the sections. 



By comparing the bodies seen here with certain of those seen 

 in preparations from cultures, the apparent forms of the para- 

 site in the lesion may be determined. 



FORMS OF ORGANISM IN TISSUE LESION 



The basic forms are simple, small, round, sometimes oval and 

 occasionally crescentic or signet-ring-shaped bodies which, unless 

 degenerated (as in fig. 10), stain intensely by Gram's and 

 general nuclear stains. Occasionally one may find within a 

 phagocyte several organisms which are bean-shaped. Some 

 are found to be slightly acid-fast. The smaller vary in size 

 from mere granules, hardly larger than the ordinary staphy- 

 lococcus (figs. 8, 9, and 14) , to bodies 3 or 4 microns in diameter 

 (figs. 8 to 14) . These show no apparent structural differentia- 

 tion. Practically all of the phagocytized parasites and the 

 smaller of those that are free are of this type. The entire-cell 

 form is produced by the development about the free-lying basic 

 forms of protoplasmic envelopes (see especially figs. 8, 9, 13, 

 and 14). In sections these bodies often measure from 5 to 6 

 microns in diameter. 



