276 The Philippine Journal of Science 1916 



In fluid media. — In plain bouillon the mycelium which is in 

 the depths remains filamentous, though the aerial growth under- 

 goes separation. After a few days the protoplasm of most of 

 the submerged filaments condenses to form small, highly refrac- 

 tile granules (figs. 43 to 45). At the same time there may be 

 formed short, irregular hyaline filaments which are faintly 

 yellow, to massing of which the brittleness and deep yellow color 

 of the tough yellow membrane which develops on banana bouil- 

 lon are due. Rarely in old bouillon cultures there appear large 

 chlamydospores (figs. 44 and 45), which develop terminally on 

 apparently undifferentiated hyphse. 



A constant feature, even in young cultures, is the produc- 

 tion of crystals which are usually flat, six-sided, and faintly 

 yellowish, though under some conditions they have the typical 

 "envelope" forms of calcium oxalate. These crystals are some- 

 times present in great numbers. 



PATHOGENICITY FOR ANIMALS 



As yet no extensive study has been made of the lesions which 

 develop after inoculation of cultures of this mycelium into labor- 

 atory animals. Suffice it for the present to say that lesions 

 not simply referable to the presence of the foreign material 

 and which contain typical tissue forms of the parasite develop 

 upon subcutaneous inoculation of fairly large doses. One rabbit 

 which was inoculated intravenously died after twenty hours. 

 There was found a peritonitis and an early plastic pericarditis, 

 and in the exudates were great numbers of a modified entire- 

 cell form of the organism. In some of the cultures from this 

 animal on ordinary media the mycelial growth did not develop, 

 while in others it appeared after three days. 



SUMMARY AND DISCUSSION 



From a case of mycotic dermatitis presenting multiform, 

 widely distributed lesions, there has been isolated on special 

 media, after several unsuccessful attempts, a fungus which is 

 thought not to have been previously described. The present 

 description is based on the observations made during its isolation 

 from the only case of this nature yet encountered and during 

 the few months since its recovery. Intensive study of numerous 

 cultures has clearly demonstrated its life history under the 

 various conditions obtaining. 



In the saprophytic, free-growing state it forms on culture 

 media a firm surface layer with abundant soft, white, loosely 

 interwoven superficial growth. Under the conditions which have 



