282 The Philippine Journal of Science i9i8 



Plate IV 



Fig. 15. From a Gram-stained smear from a primary culture on ordinary 

 medium. Numerous fine, Gram-positive granules are division 

 forms of the parasite. X 1000. 



16. Similar to above, from another culture. X 700. 



17. One appearance of an old, thin, gelatinous form, after Gram's 



stain. X 400. 



18. A typical entire-cell form from culture in smeared blood. Giemsa's 



stain. X 1000. 



19. Above, an entire-cell form v^'ith one refractile spore-granule in 



nucleus. Below, a later stage, nucleus indistinguishable, the 

 whole containing many such granules. Field slightly out of 

 focus. Giemsa's stain. X 1000. 



20. Large, soft cell about to liberate group of spores. X 1000. 



21. Unusually compact mass of spores. On account of plane of focus 



all but one appear black. Giemsa's stain, x 1000. 



22. Debris in smear from culture, containing myriads of minute, free 



spore-granules, stained light blue in the preparation. A number 

 of the larger are clearly discernible. In the center is a darkly 

 stained oval body which corresponds to the basic foi-m. Loeffler's 

 methylene blue. X 825. 



23. Group of spores beginning to develop. From culture in smeared 



blood. Giemsa's stain. X 1000. 



24. Intermediate form containing large metachromatic granules. 



Developed from spores in smeared blood. Loeffler's methylene 

 blue. X 1000. 



25. Older protozoonlike cell. Giemsa's stain. X 1000. 



26. Early stage of gelatinous body, developing from basic form. 



Giemsa's stain. X 750. 



27. Intermediate stage of gelatinous body about to differentiate basic 



forms within itself. Giemsa's stain. X 400. 



28. Group of basic forms produced within a gelatinous body. X 400. 



Plate V 



Fig. 29. Mass of gelatinous bodies, the protoplasm of which has condensed 

 to form numerous basic forms of various sizes. Somewhat earlier 

 stage than in fig. 27. X 200. 



30. Large body, from ten-day culture on banana cylinder, containing 



ordinary round basic forms and bizarre forms of similar material, 

 apparently representing an attempt at mycelial growth. X 500. 



31. A group of collapsed walls of banana-pulp cells, in smear of material 



planted on banana cylinder. These might be mistaken for vesi- 

 cular forms of the gelatinous bodies, x 100. 

 Figs. 32 and 33. Empty banana-pulp cells. X 400. 



Fig. 34. Segments of aerial hyphae, from free-growing culture on agar. 

 Tendency to deeper staining at ends. Refractile granules in one 

 segment. X 750. 

 35. Same as above, several cells showing metachromatic granules 

 (black) . X 750. 



Plate VI 



Fig. 36. Five-day growth on banana cylinder, illustrating soft, tangled 

 character of aerial grovrth. 



