X, A, 1 Pratt : Papain 



Table IX. — Analysis of Ceylon papain, sample 



11 



No. of 

 flask. 



Milk. 



Water. 



Papain 

 solution. 



Papain. 



Protein 

 undi- 

 gested. 



Protein 

 digested. 



Protein 

 digested. 



Parts of 

 protein 

 digested 

 by 1 part 

 papain. 



1 

 2 

 3 

 4 

 5 

 6 

 7 

 8 

 9 

 10 



CO. 

 25 

 25 

 25 

 25 

 25 

 25 

 25 

 25 

 25 

 25 



cc. 



23 



21 



19 



17 



15 



13 



11 



9 



7 



5 



cc. 



2 



4 



6 



8 



10 



12 



14 



16 



18 



20 



7ng. 

 10 

 20 

 30 

 40 

 50 

 60 

 70 

 80 

 90 

 100 



mg. 

 1,179 

 1,144 

 1.155 

 1,129 

 1,106 

 1,134 

 1,097 

 1,100 

 1,073 

 1,036 



mg. 



'Per cent. 





35 

 23 

 50 

 73 

 45 

 82 

 79 

 106 

 143 



3.0 

 2.0 

 4.2 

 6.2 

 3.8 

 7.0 

 6.7 

 9.0 

 12.2 



1.8 

 0.7 

 1.2 

 1.4 

 0.8 

 1.2 

 1.0 

 1.2 

 1.2 



Protein in blank = 1,179 milligrams. 



Starch present in large amount. 



Acidity of papain solution as HC1 = 0.001 per cent. 



Regarding this sample Mead Johnson & Co. states that it is a good specimen of the 

 product usually handled by drug brokers. The above analysis shows how little value it 

 actually possesses. 



No. 8. Mexican papain. — This sample was obtained from Mead 

 Johnson and Company. It was approximately the color of sam- 

 ple 6, and showed a comparatively uniform composition. The 

 results of the analysis are shown in Table X. 



Table X. — Analysis of Mexican papain, sa7nple 8. 



No. of 

 flask. 



Milk. 



Water. 



Papain 

 solution. 



Papain. 



Protein 

 undi- 

 gested. 



Protein 

 digested. 



Protein 

 digested. 



Parts of 

 protein 

 digested 

 by 1 part 

 papain. 





ce. 



ee. 



cc. 



mg. 



mg. 



mg. 



Per cent. 





1 



25 



23 



2 



10 



1,090 



129 



6.5 



12.9 



2 



25 



21 



4 



20 



1,009 



210 



17.2 



10.5 



3 



25 



19 



6 



30 



956 



263 



21.6 



8.8 



4 



25 



17 



8 



40 



889 



330 



27.1 



8.3 



5 



25 



15 



10 



50 



804 



415 



34.0 



8.3 



6 



25 



IS 



12 



60 



772 



447 



36.7 



7.5 



7 



26 



11 



14 



70 



734 



485 



39.8 



6.9 



8 



25 



9 



16 



80 



698 



521 



42.7 



6.6 



9 



26 



7 



18 



90 



660 



559 



45.8 



6.2 



10 



25 



5 



20 



100 



645 



574 



47.1 



5.7 



Protein in blank = 1,219 milligrams. 

 Starch absent. 



Acidity of papain solution as HC1 = 0.005 per cent. 



Mead Johnson & Co. states that this is unusually dark colored for Mexican gum, which 

 should more resemble the West Indian product. 



