12 



TJir Philippine Jcun-nal of Scirvrc 



1916 



No. 9. West Indian papain. — This sample was obtained from 

 Mead Johnson and Company. It was a light biscuit color, readily 

 friable, and of uniform appearance. The analysis is given in 

 Table XI. 



Table XI — A)talyiiis of Went Indian papain, samjtlc !). 



No. of 

 flMk. 



Milk. Water. 



1 



ee. 



„. ! 



25 



28 



25 



21 



25 



19! 



25 



17 



25 



15 



26 



IS 1 



26 



11 



26 



9 



25 



7 



25 



5 



I Papain i 

 ' solution. 



Papain. 



Protein 



undi- 



seated. 



mg. 



mg. 



10 



815 



20 



897 



i 80 



598 



i 40 



468 



1 60 



416 



! 60 



343 



Protein 

 digetteti. 



mg 



Protein 

 (litrested. 



I ParUof 

 protein 

 digested 

 by 1 part 

 papain. , 



20 



70 



80 



90 



100 



295 

 271 

 261 

 243 



Protein in blank = 1,215 millieranu. 

 Starch absent. 



.\cidity of papain solution as HC1 = 0.005 per cent. 



Mead Johnson & Co. states that it regards West Indian gum a.-^ the l>est produced, and 

 this view is clearly sulistantiatcd by the above analysis. 



The results of the above analyses are plotted in fig. 1, where 

 the various curves show not only the percentage of total protein 

 digested by increasing amounts of papain, but also give graphic- 

 ally a clear idea of the marked differences existing in the pro- 

 teolytic activity of the samples, whose numbers are given. 

 Thus, the amount of milk protein digested in thirty minutes at 

 40° by 1 milligram of West Indian papain is seen to exceed that 

 digested under similar conditions by 100 milligrams of sample 

 7, representing ordinary Ceylon papain. 



PREPARATION OF PAPAIN 



Various methods for converting fresh latex into dry papain 

 were investigated, in order to ascertain the extent of deteriora- 

 tion during drying and the possibility of preparing papain 

 commercially which would exceed the West Indian product in 

 its proteolytic activity. The fresh latex for this work was 

 obtained under my personal supervision. 



Green fruits were .scarified with steel knives in the usual 



