30 



The Philipinne JoiDnal of Science 



leis 



Table XXVIII. — Activity of various sampUn of papain. 



Sample 

 No. 



Souree. 



Unknown 



Ceylon . . , . , 



do 



do 



do 



do 



do 



Mexico .. 



West Indies 



FrcBh latex 



Philippine, sundried 



Philippine, alcohol 



itation 



AetiTitj I 

 number. ' 



precil>- 



0.0 



ai 



B.« 

 9.< 



6.7 

 22.0 



L8 

 12.> 



40. U 

 45.8 

 45.4 



72.2 



OTHER ENZYMES IN PAPAYA LATEX 

 Fresh papaya latex was investigated to deteiinine whether 

 enzymes other than papain were present. Flasks containing 

 the following mixtures were incubated for twenty-four hours 

 at 40^. The solutions were made neutral to phenolphthalein 

 before incubation. 



1. Active control. 



Latex 



e- 



1.0 



Water, distilled 



cc. 



50.0 



Toluol 



cc. 



0.5 



2. BoUed control. 







Latex, boiled 1 minute 



K- 



1.0 



Water, distilled 



cc. 



50.0 



Toluol 



cc. 



0.5 



S. Active extract (made in triplicate). 







Latex 



g- 



1.0 



Sucrose 



K- 



0.5 



Water, distilled 



cc. 



50.0 



Toluol 



cc. 



0.5 



No invert sugar was pi*oduced in any of these experiments, 

 thus showing the absence of invertase. 



.4. Active control. 



Latex 



K- 



1.0 



Wat«r, distilled 



cc. 



50.0 



Toluol 



cc. 



0.5 



5. Boiled control. 







Latex, boiled 1 minute 



g- 



1.0 



Water, distilled 



cc. 



50.0 



Toluol 



cc. 



0.5 



»?. Active extract (made in triplicate). 







Latex 



K- 



1.0 



Starch solution, 1 per cent 



cc. 



50.0 



Toluol 



cc. 



0.5 



