128 



The Philippine Journal of Srii )ue 



191S 



Albuniinases are probably absent in cacao or, at least, so feebly 

 active as to be negligible in so far as their influence on the fer- 

 mentation may be concerned. 



Table V. — Tests for protease. 

 SERIES 1. 







Cc. N/10. 



20 cc. 



' 20 cc. 

 20 cc. 

 20 cc. 



water, 10 cc. pulp extract. 48 hours at 38° C 



ao6 



peptone solution. 10 cc. pulp extract, 48 hourB at 38° C 



2.20 



peptone solution. 10 cc. pulp extract heated, 48 hours at 38° C 



L20 



peptone solution, 10 cc. water, 48 hours at 38° C 



1.06 







Tryto- 



phane 



test. 



SERIES 2. 



20 cc. water, 10 cc. seed extract. 48 hours at 38- C 



20 cc. peptone solution. 10 cc. seed extract, 48 hours at 38° C 



20 cc. peptone solution, 10 cc seed extract heated, 48 hours at S8° C. 



0.06 

 LIO 



i.oe 



SERIES 3. 



20 cc. water. 10 cc. fermented seed extract. 74 hours at 38- C 



20 cc. peptone solution. 10 cc. fermented seed extract, 74 hours at 88° C 



20 cc. peptone solution. 10 cc. fermented seed extract heated. 74 hours at 38° C. 



0.06 

 2.00 

 1.10 



The peptone solution used was a 2 per cent solution of Witte 

 peptone in water. 



Judging from the tryptophane test and the difference in number 

 of cubic centimeters of NaOH required for the neutralization of 

 the active samples and the controls, the pulp and fermented seed 

 extracts contain a proteolytic enzyme which reacts with peptone. 

 It appears to be absent in the seed extract. 



Table VI. — Tests for inulase. 

 SERIES 1. 



Test for 



(glucose. 



11 CC. water, 10 cc. pulp extract. 70 hours at 38° C 



10 cc. water, 10 cc. pulp extract. 1 cc. inulin solution, 70 hours at 38^ C - 



10 cc. water, 10 cc. pulp extract heated, 1 cc. inulin solution, 70 hours at 88° C, 

 20 cc. water, 1 cc. inulin solution, 70 hours at 38^ C. 



SERIES 2. 



11 cc. water. 10 cc. seed extract, 70 hours at 38^ C - 



10 cc. water, 10 cc. seed extract, 1 cc. inulin solution, 70 hours at 38° C 



10 cc. water, 10 cc. seed extract heated. 1 cc. inulin solution, 70 hours at 38° C 



++ 

 + + 



