X, A, 2 



Brill: Enzymes of Cacao 



129 



Table VI. — Tests for inulase — Continued. 



SERIES 3. 



11 cc. water, 10 cc. fermented seed extract, 70 hours at 38° C 



10 cc. water, 10 cc. fermented seed extract, 1 cc. inulin solution, 70 hours at 38° C 



10 cc. water, 10 cc. fermented seed extract heated, 1 cc. inulin solution, 70 hours at 38° C. 



Test for 

 glucose. 



4- 



The inulin solution used was a 10 per cent solution in water. 

 The test for glucose carried out showed that no hydrolysis of 

 inulin had taken place, thus proving the absence of inulase. 



Table VII. — Tests for reductase. 

 SERIES 1. 



Color 

 change. 



10 cc. water. 10 cc. pulp extract, 1 cc. methylene blue solution, 24 hours at 38° C 



10 cc. water. 10 cc. pulp extract heated, 1 cc. methylene blue solution, 24 hours at 38° C 

 20 cc. water, 1 cc. methylene blue solution, 24 hours at 38° C ^ 



SERIES 2. 



10 cc. water, 10 cc. seed extract, 1 cc. methylene blue solution, 24 hours at 38° C 



10 cc. water, 10 cc. seed extract heated, 1 cc. methylene blue solution, 24 hours at 38° C 



+ + 

 + + 



+ + + 

 + - + 



SERIES 3. 



10 cc. water, 10 cc. fermented seed extract, 1 cc. methylene blue solution, 24 hours at 

 38° C -- -._ 



10 cc. water, 10 cc. fermented seed extract heated, 1 cc. methylene blue solution, 24 

 hours at 38° C 



+ + + 

 + -I- + 



The methylene blue solution was made by saturating 1 cubic 

 centimeter of alcohol with methylene blue and diluting the result- 

 ing solution to 40 cubic centimeters with water. In each case 

 the change in color for the control is the same as the change in 

 the fresh sample, indicating that there is no reductase present. 



Table VIII. — Tests for oxidases. 

 SERIES 1. 





Change in color noted at 38° C. 



Initial. 



End of- 



i hour. 



4 hours. 



24 hours. 



20 cc. water, 1 cc. guaiacum tincture. 



+ 



+ -)- 





10 cc. water, 10 cc. pulp extract, 1 cc. guaiacum tincture. 

 10 cc. water, 10 cc. pulp extract heated, 1 cc. guaiacum 



+ + + 







