130 



Tlw Pliilippinc Jovniol of Science 



1910 



Table VIII. — TcxtK for oxidanea — Continued. 

 SERIES 2. 



Chanirc in color noti-d at IJK-' C. 



I'Jnd uf 

 Initial. I - 



i hour. 4 hours. 24 hourt. 



20 cc. water. 10 cc. seed extract, 1 cc. iruaiacum tincture.! ++ ( + + + +++ i 

 20 cc. water, 10 cc. seed extract heated, 1 cc. iruaiacum ' , 



tincture ' — , _ _ ( _ 



1 1 I 



SERIES 8. 



20 cc. water, in cc. fermented seed extract, 1 cc. truaia- 

 cum tincture 



20 cc. water, lu cc. fermented seed extract heated, 1 cc. 

 eruaiacum tincture 



+ 



++ 



++ 



+ 



The 20 per cent g-uaiacum tincture used here was made by 

 grinding- the gum in alcohol and allowing it to stand at room 

 temperature for two days. At the end of this time the solution 

 was filtered and used at once. 



Oxidases exist in the fresh .^eed in large quantities, but are 

 present in relatively large quantities in the pulp also, although 

 considerably less than in the seed. They exist in comparatively 

 small amounts in the fermented seed, as evidenced by the changes 

 in color recorded above. When the seeds are ground in water, 

 the solution very soon assumes a brown color, and a flocculent 

 precipitate separates out. This mass is the tannin precipitated 

 by the oxidases. 



Table IX. — Tentx for diastase. 



SERIES 1. 



40 cc. water. 10 cc. pulp extract, 48 hours at 38° C 



40 cc. starch solution, 10 cc. pulp extract, 48 hours at 38° C 



40 cc. starch solution, 10 cc. pulp extract heated, 48 hours at 38° C 

 40 cc. starch solution, 10 cc. water. 48 hours at 38° C 



SERIES 2. 



40 cc. water, 10 cc. seed extract. 48 hours at 38° C — 



40 cc. starch solution, 10 cc. seed extract. 48 hours at 38° C 



40 cc. starch solution. 10 cc. seed extract heated, 48 hours at 38° C . 



Test for— 



Glucoee. Starch, 



+ + 

 + H- 

 ++ 



++ + + 

 + + ++ 

 + + + + 



+ + + + 

 + + + + 



