132 



The Philippiiu- Jnunial of Science 



i'.nb 



A 10 per cent cane-sugar solution was used for the detection 

 of invertase. 



The test for invertase was strongly positive in the pulp extract ; 

 a fair test resulted in the fermented seed extract, while a negative 

 test was obtained in the seed extract. 



Table XI. —Teats for raffinase. 

 SERIES 1. 



10 cc. waujr. 10 cc. pulp extract. 72 hours at 38^ C , 



10 cc. raffinoee solution, 10 cc. pulp extract. 72 hours at 38^ 



10 cc. raffinose solution, 10 cc. pulp extract heated. 72 hours at 3*!^ C 

 10 cc. raffinose solution, 10 cc. water. 72 hours at 88° C 



SERIES 2. 



10 cc. water, 10 cc. seed extract, 72 hours at 38^ C 



10 cc. raffinose solution, 10 cc. seed extract, 72 hours at 38° C 



10 cc. raffinose solution. 10 cc. seed extract heated, 72 hours at 38° C 



Ventzke 

 scale. 



0.6 



5.6 



11.0 



11.0 



0.0 

 +10.9 

 +U.6 



SERIES 3. 



10 cc. water. 10 cc. fermented seed extract, 74 hours at 38° C 



10 cc. raffinose solution, 10 cc. fermented seed extract, 71 hours at 38° C 



10 cc. raffinose solution. 10 cc. fermented seed extract heated, 74 hours at 38° C- 



0.0 



< 9.8 

 + 1L3 



A 5 per cent solution of raffinose was used in this experiment. 

 Any hydrolysis of the raffinose by raffinase would decrease the 

 polariscope reading. There is a considerable decrease of the 

 reading for the fresh pulp extract from the reading of the controls 

 used and a slight difference in the reading of the analogous 

 solution in series 2 and 3, indicating the presence of considerable 

 activity of the raffinase in series 1 and less activity in series 2 

 and 3 ; or, the pulp contains a raffinase in considerable quantities, 

 the fermented seed in somewhat less quantities, while the fresh 

 seed shows scarcely any activity toward raffinose. 



Table XII. — Testst for maltase. 

 SERIES 1. 



Ventzke 

 scale. 



10 cc. water, 10 cc. pulp extract. 72 hours at 38° C 



10 cc. maltose solution, 10 cc pulp extract, 72 hours at 38° C 



10 cc. maltose solution. 10 cc. pulp extract heated, 72 hours at 38° C 

 10 cc. maltose solution, 10 cc. water. 72 hours at 3S° C , 



o 

 + 0.6 

 +23.4 

 +23.7 

 +23.4 



