X, B, 1 Ruediger: Preparation of Tetanus Antitoxin 33 
September 11, 1911, another injection of 750 cubic centimeters 
was given. The serum was tested for antitoxin and was found 
to contain 150 units per cubic centimeter. On September 20, 
1911, the horse was bled 3 liters and three days later was bled 
2 liters. 
Beginning with October 2, 1911, the injections were given at 
intervals of a week and the doses were increased more gradually. 
A test sample of blood taken in the latter part of October con- 
tained 350 units of antitoxin per cubic centimeter of serum. On 
November 1, 1911, the horse was bled 3 liters. The injec- 
tions were continued at intervals of a week and on November 
30, 1911, 3 liters of blood were withdrawn. The serum con- 
tained 500 units of antitoxin per cubic centimeter. After this, 
the antitoxin content in the serum rose more slowly, testing 
600 units per cubic centimeter on December 31, 1911, and the 
_ horse had lost much flesh. Still expecting to drive the antitoxin 
content higher, the injections were continued; but instead of 
rising, the antitoxin content began to fall. On January 25, 
1912, the horse was bled 3 liters and the serum tested 500 units 
per cubic centimeter. 
This horse received a total of 8,856 cubic centimeters of toxin, 
was bled 14 liters, and furnished about 5,600 cubic centimeters 
of serum, or 1,920,000 units of antitoxin. Deducting 50 per 
cent as allowance made for deterioration and accidental losses, 
we can count on marketing 960,000 units (see figs. 1 to 9, charts 
1—A to 1-I, for horse 1). 



















GAM TeTANus Nha, fo B 
JUNE 1) ES 3,4] 51647 8} 7 |/O} ss 112413 14 | 19 416 | /7 4/8 11/9 420 4 21 (22123 124 [25126 127128127430 
°C. 
37 
=a 5 5 5 5 ae 3 z 
3’ ; So (ey S S 2 °) 
RS & il 4 5 os S 4 
ae 3 Gel > be) oO] w Nw os 
Fic. 1. Temperature chart of horse 1 for June, 1911. 
191] TETANus No.1 8 
Juiy}/J2]3 4,4) 5) 647} 849 | sel i} s2[13 [4 s5j fe) 7418 1/9 420121 [22423 [ay jasiac j27\25 [27 | 40131 
°6. 







38 





37 
1/0, 
wy 
~ 
300, 
400, 
o s Ss s 2 
x 2 = 2 SI 
Fig. 2. Temperature chart of horse 1 for July, 1911. 
132195——3 
