36 The Philippine Journal of Science 1915 
and 1,000 units on August 1, 1911. The injection of toxin was 
begun with 0.01 cubic centimeter filtrate given at intervals of 
three or four days, and the doses were rapidly increased. During 
the first three months 21 injections were given, and at the last 
injection 1,000 cubic centimeters of toxic filtrate were given. 
On September 27, 1911, the serum was tested; it contained less 
than 50 units of antitoxin per cubic centimeter. 
After September the doses were reduced and the injections 
were given at intervals of a week. On November 10, 1911, the 
horse was bled 3 liters, and the serum contained 75 units per 
cubic centimeter. Three liters of blood were withdrawn on 
December 21, 1911; the serum obtained tested 100 units per 
cubic centimeter. The injections were continued at intervals 
of a week; on February 23, 1912, the horse was bled 4 liters, 
and on February 29, 1912, it was bled 5 liters. The serum 
contained 150 units per cubic centimeter. 
Horse 2 received a total of 8,806 cubic centimeters of tetanus 
toxin; it was bled 15 liters and produced about 6 liters of anti- 
toxic serum or 750,000 units of antitoxin. Deducting 50 per 
cent of antitoxin as allowance made for deterioration and other 
losses, we have left 375,000 units for marketing. 
At this time there was very little demand for antitetanic serum 
or the horses would have been bled more, as will be seen later 
(see figs. 10 to 18, charts 2—A to 2-I, for horse 2). 
Tetanus ee 
~ 
a it 
a EESEEEEE | 
PREC 
SEZERERER 
§ 780.0 Pig titty 







= 
= 
= 
Ty 
a 
= 
4 
= 
EI 
= 
= 
= 
. 
° 
8, 
“ 
Fic. 11. Temperature chart of horse 2 for August, 1911. 
