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Fic. 27. Chart showing the antitoxin curve for horse 3. Units per cubic centimeter by months. 
HORSES 4 AND 5 
On June 1, 19138, the immunization of horses 4 and 5 was 
begun. Each horse was fortified with 2,500 units of tetanus 
antitoxin, 1,500 units being given on June 1, 1918, and 1,000 
units on July 7, 1913. Beginning with a dose of 2 cubic centi- 
meters, injections of toxin were made at intervals of one week. 
The doses were increased to 1,000 cubic centimeters in four 
months and one week. The serums contained 75 units of anti- 
toxin per cubic centimeter, and each horse was bled; horse 4 
was bled 8 liters, and horse 5, 10 liters. After this bleeding 
smaller doses of toxin were given. In the latter part of No- 
vember, 1913, the serum of horse 4 contained 300 units per cubic 
