4? The Philippine Journal of Science 1915 
centimeters and 11 liters of blood were withdrawn, about 40 
per cent of which was serum. Horse 5 was bled 12 liters in 
the first week of December, 1913, which yielded about 40 per 
cent of serum that contained 300 units of antitoxin per cubic 
centimeter... The injections of toxin were again continued. On 
January 21, 1914, horse 4 was bled 6 liters; the serum contained 
300 units of antitoxin per cubic centimeter. Horse 5 was bled 
10 liters—5 liters on January 23, 1914, and 5 liters on January 
27, 1914. The serum obtained contained 250 units per cubic 
centimeter. In the middle of March, 1914, the serum of horse 
4 contained 300 units of antitoxin per cubic centimeter and the 
horse was bled 8 liters. The serum of horse 5 contained 350 
units of antitoxin per cubic centimeter, and 10 liters of blood 
were withdrawn on March 23. Horse 4 received one more in- 
jection of toxin and was bled to death on March 31, 1914. At 
the last bleeding 14 liters of blood were obtained which yielded 
about 40 per cent of serum, testing 225 units per cubic centi- 
meter. The antitoxin content in the serum of horse 4 reached 
its highest recorded mark in the sixth month and was maintained 
until death, ten months after the beginning of immunization. 
_ Horse 4 received in all 14,633.5 cubic centimeters of tetanus 
toxin and was bled 47 liters. It produced about 18.8 liters of 
antitetanic serum or 4,500,000 units of tetanus antitoxin. De- 
ducting from the antitoxin 50 per cent as allowance made for 
deterioration and for accidental losses, we have left 2,250,000 
units for marketing (see figs. 28 to 38, charts 4-A to 4—K, 
for horse 4). 
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